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Anti-Human CD223 (LAG-3) Super Bright 436

Also known as: Lymphocyte Activation Gene-3

Clone: 3DS223H

RUO: For Research Use Only. Not for use in diagnostic procedures.

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SKU# 62-2239

Cat. No. Size
62-2239-41 25 tests
62-2239-42 100 tests

Data for Anti-Human CD223 (LAG-3) Super Bright 436.

Staining of 3-day Anti-Human CD3 and CD28 Functional Grade Purified (cat. 16-0037 and...View More

  • Data for Anti-Human CD223 (LAG-3) Super Bright 436.
Description

Description: This 3DS223H monoclonal antibody recognizes human CD223 also known as Lymphocyte Activation Gene 3 (LAG-3). LAG-3 is a 70-kDa surface glycoprotein belonging to the Ig superfamily with homology to CD4. LAG-3 binds to MHC class II with higher affinity than CD4 and is thought to be involved in the negative regulation of T cell activation and homeostatic proliferation. Surface expression of LAG-3 has been reported on activated T cells (including regulatory T cells) and NK cells. CD8+ T cells usually express LAG-3 at significantly higher levels than CD4+ T cells. Coexpression of LAG-3 and CD49b has been proposed to identify human and mouse Type 1 regulatory T cells (Tr1 cells).

This 3DS223H antibody will recognize a formaldehyde-fixed epitope.

Details
Host Mouse
Isotype IgG1, kappa
Reactivity Human
Conjugate Super Bright 436
Laser Violet Laser
Emit 436 nm
Excite 405 nm
Legal Patent pending.
Reported Applications Flow Cytometric Analysis
Documentation
TDS Link Download TDS
Additional Formats
Cat. No. Name Excite Emit Application Reg.
17-2239 Anti-Human CD223 (LAG-3) APC 633 - 647 nm 660 nm FC RUO
48-2239 Anti-Human CD223 (LAG-3) eFluor® 450 405 nm 445 nm FC RUO
11-2239 Anti-Human CD223 (LAG-3) FITC 488 nm 520 nm FC RUO
47-2239 Anti-Human CD223 (LAG-3) APC-eFluor® 780 633 - 647 nm 780 nm FC RUO
25-2239 Anti-Human CD223 (LAG-3) PE-Cyanine7 488 - 561 nm 775 nm FC RUO
46-2239 Anti-Human CD223 (LAG-3) PerCP-eFluor® 710 488 nm 710 nm FC RUO
12-2239 Anti-Human CD223 (LAG-3) PE 488 - 561 nm 578 nm FC RUO
61-2239 Anti-Human CD223 (LAG-3) PE-eFluor® 610 488 - 561 nm 607 nm FC RUO
Related Products
Cat. No. Name Excite Emit Application Reg.
00-6993 7-AAD Viability Staining Solution FC GPR
16-0037 Anti-Human CD3 Functional Grade Purified FC, FA RUO
62-4714 Mouse IgG1 K Isotype Control Super Bright 436 405 nm 436 nm FC RUO
11-0087 Anti-Human CD8a FITC 488 nm 520 nm FC RUO
16-0289 Anti-Human CD28 Functional Grade Purified FC, FA RUO
SB-4400 Super Bright Staining Buffer FC GPR
References

References: Ballke C, Gran E, Baekkevold ES, Jahnsen FL. Characterization of Regulatory T-Cell Markers in CD4+ T Cells of the Upper Airway Mucosa. PLoS One. 2016 Feb 11;11(2):e0148826. (3DS223H, FC, PubMed)

Long AH, Haso WM, Shern JF, Wanhainen KM, Murgai M, Ingaramo M, Smith JP, Walker AJ, Kohler ME, Venkateshwara VR, Kaplan RN, Patterson GH, Fry TJ, Orentas RJ, Mackall CL. 4-1BB costimulation ameliorates T cell exhaustion induced by tonic signaling of chimeric antigen receptors. Nat Med. 2015 Jun;21(6):581-90 (3DS223H, FC).

Gagliani N, Magnani CF, Huber S, Gianolini ME, Pala M, Licona-Limon P, Guo B, Herbert DR, Bulfone A, Trentini F, Di Serio C, Bacchetta R, Andreani M, Brockmann L, Gregori S, Flavell RA, Roncarolo MG. Coexpression of CD49b and LAG-3 identifies human and mouse T regulatory type 1 cells. Nat Med. 2013 Jun;19(6):739-46.

Camisaschi C, Casati C, Rini F, Perego M, De Filippo A, Triebel F, Parmiani G,Belli F, Rivoltini L, Castelli C. LAG-3 expression defines a subset of CD4(+)CD25(high)Foxp3(+) regulatory T cells that are expanded at tumor sites. J Immunol. 2010 Jun 1;184(11):6545-51.

Joosten SA, van Meijgaarden KE, Savage ND, de Boer T, Triebel F, van der Wal A, de Heer E, Klein MR, Geluk A, Ottenhoff TH. Identification of a human CD8+ regulatory T cell subset that mediates suppression through the chemokine CC chemokine ligand 4. Proc Natl Acad Sci U S A. 2007 May 8;104(19):8029-34.

Maçon-Lemaître L, Triebel F. The negative regulatory function of the lymphocyte-activation gene-3 co-receptor (CD223) on human T cells. Immunology.2005 Jun;115(2):170-8.


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