CD22 PerCP-eFluor® 710

Description: The S-HCl-1 monoclonal antibody recognizes human CD22 (Siglec-2), which is a member of the siglec subgroup of the Ig superfamily. CD22 is a type I transmembrane glycoprotein composed of two polypeptide chains, CD22 alpha and CD22 beta, 130 and 140 kDa respectively, produced by alternative splicing of the CD22 gene. CD22 is expressed at high levels on mature B cells. The extracellular portion of CD22 contains seven Ig-like domains, some of which are capable of binding ligands with sialic acid moieties expressed on epithelial, endothelial, B and T cells. The intracellular portion of CD22 contains 6 tyrosine residues contained within immunotyrosine-based inhibitory motifs (ITIM) and immunotyrosine-based activation-like motifs (ITAM), which are phosphorylated upon B-cell receptor engagement and enable CD22 to participate in the positive and negative regulation of B-cell receptor signaling.

References: Procedures for the Collection of Diagnostic Blood Specimens by Venipuncture (H3-A6), 3rd Edition published by the National Committee for Clinical Laboratory Standards.

Nitschke L. The role of CD22 and other inhibitory co-receptors in B-cell activation. Curr Opin Immunol. 2005 Jun;17(3):290-7.

Campana D, Janossy G, Bofill M, Trejdosiewicz LK, Ma D, Hoffbrand AV, Mason DY, Lebacq AM, Forster HK. Human B cell development. I. Phenotypic differences of B lymphocytes in the bone marrow and peripheral lymphoid tissue. J Immunol. 1985 Mar;134(3):1524-30.

Nadler L. B Cell/Leukemia Panel Workshop: Summary and comments. In: Reinherz EL, Haynes BF, Nadler LM,Bernstein ID, eds. Leukocyte Typing II: Human T Lymphocytes. New York: Springer-Verlag; 1986:3-43.