CD16 PE-Cy7

Also known as: Low Affinity IgG Receptor 3

Clone: CB16

Analyte Specific Reagent (ASR): Analytical and performance characteristics are not established.

SKU# 8025-0168

Cat. No.	Size
8025-0168-025	25 tests
8025-0168-120	120 tests
Please inquire about custom configurations or bulk packaging.

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Fluorescence profiles of normal human peripheral blood lymphocytes unstained (blue histogram) or...View More

Description

Description: The CB16 monoclonal antibody recognizes CD16 (Fc gammaRIII), the low-affinity receptor for IgG with an apparent molecular weight of 50-80 kDa. CD16 is represented by two similar genes, CD16A (Fc gammaRIIIA), which exists as a hetero-oligomeric polypeptide-anchored form in macrophages and NK cells and CD16B (Fc gammaRIIIB), which exist as a monomeric GPI-anchored form in neutrophils. Furthermore, there are two known polymorphisms of CD16B, NA-1 and NA-2. Individuals homozygous for NA-2 show a lower phagocytic capacity compared with NA-1. CD16 binds IgG in the form of immune complexes and shows preferential binding of IgG1 and IgG3 isotypes and minimal binding of IgG2 and IgG4. Upon IgG binding, both CD16 isoforms initiate signal transduction cascades that lead to a variety of responses including antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, degranulation and proliferation.

Details

Reactivity	Human
Conjugate	PE-Cyanine7
Laser	Blue Laser
Emit	760 nm
Excite	488 nm
Legal	For research (not to include in vivo research) and/or in vitro diagnostic use only. The only permitted commercial use is provision of research (not to include in vivo research) services and/or in vitro diagnostic services. Not for therapeutic or in vivo applications. Other use needs licenses which are available from GE Healthcare Bio-Sciences Corp. Under U.S. patent nos. Nos 5,268,486, 5,569,587 and 5,627,027 and foreign equivalents and pending applications. This product is subject to proprietary rights of GE Healthcare Bio-Sciences Corp. (“GE Healthcare”) and Carnegie Mellon University (“CMU”) and made and sold under license from GE Healthcare. The product bearing this label is licensed for sale only for research (not to include in vivo research) and in addition may be used to perform research (not to include in vivo research) and/or in vitro diagnostic services for third parties. It is not licensed nor are there any implied licenses for any other use including, without limitation, commercial uses unless expressly authorized by GE Healthcare in writing. If you intend to use this product commercially and you do not have a license to use this product for the intended commercial purpose, return this product, unopened to eBioscience, Inc. 10255 Science Center Drive, San Diego, California 92121 USA and any money paid for the product will be refunded.
Reported Applications	Flow Cytometric Analysis

Documentation

TDS Link	Download TDS

Additional Formats

Cat. No.	Name	Excite	Emit	Application	Reg.
8046-0168	CD16 PerCP-eFluor® 710	488 nm	710 nm	FC	ASR
8048-0168	CD16 eFluor® 450	405 nm	450 nm	FC	ASR
8012-0168	CD16 PE	488 nm	575 nm		ASR
8011-0168	CD16 FITC	488 nm	518 nm		ASR

References

References: Deaglio S, Zubiaur M, Gregorini A, Bottarel F, Ausiello CM, Dianzani U, Sancho J, Malavasi F. Human CD38 and CD16 are functionally dependent and physically associated in natural killer cells.Blood. 2002 Apr 1;99(7):2490-8.

Wirthmueller U, Kurosaki T, Murakami MS, Ravetch JV. Signal transduction by Fc gamma RIII (CD16) is mediated through the gamma chain.J Exp Med. 1992 May 1;175(5):1381-90.

View More Products

Antigens	Cell Type	Formats
CD16	Macrophage & Monocyte Cells NK & NKT Cells	PE-Cyanine7