Anti-Human CD16 Functional Grade Purified

Description: The eBioCB16 monoclonal antibody recognizes CD16 (FcγRIII), the low-affinity receptor for IgG with an apparent molecular weight of 50-80 kDa. CD16 is represented by two similar genes, CD16A (FcγRIIIA), which exists as a hetero-oligomeric polypeptide-anchored form in macrophages and NK cells and CD16B (FcγRIIIB), which exist as a monomeric GPI-anchored form in neutrophils. Furthermore, there are two known polymorphisms of CD16B, NA-1 and NA-2. Individuals homozygous for NA-2 show a lower phagocytic capacity compared with NA-1. CD16 binds IgG in the form of immune complexes and shows preferential binding of IgG1 and IgG3 isotypes and minimal binding of IgG2 and IgG4. Upon IgG binding, both CD16 isoforms initiate signal transduction cascades that lead to a variety of responses including antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, degranulation and proliferation.

References: Deaglio S, Zubiaur M, Gregorini A, Bottarel F, Ausiello CM, Dianzani U, Sancho J, Malavasi F. Human CD38 and CD16 are functionally dependent and physically associated in natural killer cells.Blood. 2002 Apr 1;99(7):2490-8. (CB16, FC, PubMed)

Zilber MT, Gregory S, Mallone R, Deaglio S, Malavasi F, Charron D, Gelin C. CD38 expressed on human monocytes: a coaccessory molecule in the superantigen-induced proliferation.Proc Natl Acad Sci U S A. 2000 Mar 14;97(6):2840-5. (CB16, Cell Separation, PubMed)

Wirthmueller U, Kurosaki T, Murakami MS, Ravetch JV. Signal transduction by Fc gamma RIII (CD16) is mediated through the gamma chain.J Exp Med. 1992 May 1;175(5):1381-90.