Anti-Human/Non-Human Primate CD123 eFluor® 450

Description: This 7G3 monoclonal antibody reacts with human and non-human primate CD123, the alpha chain of the IL-3 receptor. This 60-70 kDa transmembrane protein binds to IL-3 with low affinity by itself, and when associated with CD131 (the common beta chain) binds IL-3 with high affinity. CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, and megakaryocytes. In addition, this molecule can be detected on CD34+CD38- hematopoietic stem cells and acute myeloid leukemic (AML) cells.

The 7G3 antibody has been demonstrated to inhibit IL-3-mediated cell proliferation and homing of AML leukemic stem cells. Crossblocking studies suggest that 7G3 recognizes a different epitope from 6H6.

The 7G3 antibody has been reported to crossreact to chimpanzee, cynomologous monkey, olive baboon, pigtailed macaque, rhesus macaque, and sooty mangabey.

References: Van Brussel I, Van Vré EA, De Meyer GR, Vrints CJ, Bosmans JM, Bult H. Expression of dendritic cell markers CD11c/BDCA-1 and CD123/BDCA-2 in coronary artery disease upon activation in whole blood. J Immunol Methods. 2010 Oct 31;362(1-2):168-75.(7G3, FC)

Jin L, Lee EM, Ramshaw HS, Busfield SJ, Peoppl AG, Wilkinson L, Guthridge MA, Thomas D, Barry EF, Boyd A, Gearing DP, Vairo G, Lopez AF, Dick JE, Lock RB. Monoclonal antibody-mediated targeting of CD123, IL-3 receptor alpha chain, eliminates human acute myeloid leukemic stem cells. Cell Stem Cell. 2009 Jul 2;5(1):31-42. (7G3, FG)

Brown KN, Barratt-Boyes SM. Surface phenotype and rapid quantification of blood dendritic cell subsets in the rhesus macaque. J Med Primatol. 2009 Aug;38(4):272-8. (7G3, FC, rhesus)

Brown KN, Trichel A, Barratt-Boyes SM. Parallel loss of myeloid and plasmacytoid dendritic cells from blood and lymphoid tissue in simian AIDS. J Immunol. 2007 Jun 1;178(11):6958-67. (7G3, FC, ICC, rhesus)

Willmann K, Dunne JF. A flow cytometric immune function assay for human peripheral blood dendritic cells. J Leukoc Biol. 2000 Apr;67(4):536-44.