Anti-Human CD101 Biotin

Description: The monoclonal antibody BB27 reacts to human CD101 also known as V7 antigen, a member of the Ig superfamily. CD101 is a disulfide linked homodimer of unknown function. Expression of CD101 is found on monocytes, granulocytes and dendritic cells (Langerhan-like cells HLA-DR, CD1a, CD1c). In addition expression on T lymphocytes is important for cell activation. CD101+ CD28+ cells are very responsive to CD28 signaling. In combination with anti-CD28 or suboptimal levels of anti-CD3, anti-CD101 can increase proliferation thereby suggesting an activating role. The monoclonal antibody BB27 has been shown to inhibit the T cell reactivity in allogeneic and antigen-specific mixed DC-T cell cultures. Recently it has been demonstrated that mouse CD101 is found on a subpopulation of regulatory T cells (CD4, CD25, Foxp3 positive) that have high suppressor activity. Expression of CD101 on human PBMCs shows staining of about 30% of the Foxp3 positive cells. Studies have not confirmed higher suppressor activity in the human CD101 population.

References: Gouttefangeas C, Jacquot S, Meffre E, Schmid M, Boumsell L, Bensussan A. Differential proliferative responses in subsets of human CD28+ cells delineated by BB27 mAb.Int Immunol. 1994 Mar;6(3):423-30. (BB27, FC, PubMed)

Bouloc A, Bagot M, Delaire S, Bensussan A, Boumsell L. Triggering CD101 molecule on human cutaneous dendritic cells inhibits T cell proliferation via IL-10 production.Eur J Immunol. 2000 Nov;30(11):3132-9. (BB27, FA, PubMed)

Bouloc A, Boulland ML, Geissmann F, Fraitag S, Andry P, Teillac D, Bensussan A, Revuz J, Boumsell L, Wechsler J, Bagot M. CD101 expression by Langerhans cell histiocytosis cells.Histopathology. 2000 Mar;36(3):229-32. (BB27, Ihf, PubMed)