Human Caspase 9 FlowCytomix Simplex

Description: This FlowCytomix Simplex Kit is designed for the measurement of Human Caspase-9 in an immunoassay analyzed on a flow cytometer. Together with the FlowCytomix Human Basic Kit (cat. BMS8420FF), this kit can be used to detect Caspase-9 alone or can be multiplexed with other Simplex Kits to measure a variety of analytes.

This kit contains bead population A7.

Caspase-9, formerly called Apaf-3, binds to Apaf-1 in the presence of cytochrome c and dATP, which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3; caspase-9 thus is the most upstream member of the apoptotic protease cascade that is triggered by cytochrome c and dATP.
Caspase-9 is essentially localized in mitochondria, the disruption of the outer mitochondrial membrane occurring early during apoptosis is critical for its subcellular redistribution and activation.
Apart from cleavage of caspase-3, the downstream enzymes caspase-6 and ?7 are targets of caspase-9 activity. Caspase-9 forms dimers in the course of activation. Cytochrome c/Apaf-1/caspase-9 form a so called apoptosome, amplifying the caspase cascade. Feedback cleavage of caspase-9 by caspase-3 again significantly increases the activity of the apoptosome.
Caspase-9 plays a crucial role in all pathologies involving apoptotic processes. Specifically the involvement of caspase-9 has been described in human gastric cancer, ovarian carcinoma, neuroblastoma, gliomas as well as in Alzheimer?s disease, Huntington?s disease and osteoarthritis.

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