Human Cardiovascular 6plex FlowCytomix Multiplex

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# BMS811/2FF*

Cat. No. Size
BMS811/2FF 96 tests
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Data for Human Cardiovascular 6plex FlowCytomix Multiplex.

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  • Data for Human Cardiovascular 6plex FlowCytomix Multiplex.
Description

Description: This Human Cardiovascular 6plex FlowCytomix Kit is designed for the measurement of human sCD40L, IL-6, IL-8, MCP-1, sP-selectin and t-PA in an immunoassay analyzed on a flow cytometer.

BMS811/2FF offers a combination of immunological markers of inflammatory processes and molecules involved in apoptotic processes together with acute phase proteins and proteins associated with tissue damage.

Details
Reactivity Human
Analyte Parameters sCD40L, IL-6, IL-8, MCP-1, sP-selectin, t-PA
Sample Volume 25 uL
Suitable Sample Types cell culture supernatant, serum, plasma (EDTA, citrate)
Sensitivity sCD40L: 24.3 pg/mL
IL-6: 0.4 pg/mL
IL-8: 1.1 pg/mL
MCP-1: 1.3 pg/mL
sP-selectin: 1.2 ng/mL
t-PA: 4.8 pg/mL
Standard Curve Range CD40L: 55 - 40,000 pg/mL
IL-6, t-PA: 27 - 20,000 pg/mL
IL-8: 13.7 - 10,000 pg/mL
MCP-1: 41 - 30,000 pg/mL
P-selectin: 3.0 - 2,000 pg/mL
Components 1 vial Setup Beads (SB)
6 vials (175 ul) Fluorescent Beads (20x) coated with specific antibodies
12 vials (2 x 6) Standard (lyophilized)
6 vials (350 ul) Biotin-Conjugate (20x) (specific antibody conjugated to biotin)
1 bottle (50 mL) Assay Buffer (10x) (PBS with 10% BSA)
1 bottle (13 mL) Reagent Dilution Buffer (RDB), ready to use
1 vial (200 ul) Streptavidin-Phycoerythrin (Streptavidin-PE)
1 96 - well Filter Plate
6 Adhesive Films
Reported Applications Multiplex Immunoassay
Documentation
TDS Link Download TDS
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References

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Kelso,A.; Troutt,A.B.; Maraskovsky,E.; Gough,N.M.; Morris,L.; Pech,M.H.; Thomson,J.A.. Heterogeneity in lymphokine profiles of CD4+ and CD8+ T cells and clones activated in vivo and in vitro. Immunol.Rev. 1991;123:85-114. (Link)

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