Anti-CD11c APC-eFluor® 780

Description: The BU15 monoclonal antibody reacts with canine, human, and non-human primate CD11c, the 150 kDa integrin aX chain. CD11c non-covalently associates with beta2 integrin to form the CD11c/CD18 heterodimer. This complex is expressed on monocytes, granulocytes, macrophages, NK, dendritic cells, and subset of T and B lymphocytes. CD11c/CD18 binds to CD54, iC3b and fibrinogen and plays a role in leukocyte adhesive interactions. CD11c also binds complement fragment iC3b to mediate phagocytosis of opsonized particles.

The BU15 antibody has been reported to crossreact to cynomologus monkey. Moreover, this antibody has been reported to exhibit blocking activity. Crossblocking studies suggest that BU15 recognizes a different epitope from clone 3.9.

References: Sadhu C, Hendrickson L, Dick KO, Potter TG, Staunton DE. Novel tools for functional analysis of CD11c: activation-specific, activation-independent, and activating antibodies. J Immunoassay Immunochem. 2008;29(1):42-57. (BU15, FA)

Sadhu C, Ting HJ, Lipsky B, Hensley K, Garcia-Martinez LF, Simon SI, Staunton DE. CD11c/CD18: novel ligands and a role in delayed-type hypersensitivity. J Leukoc Biol. 2007 Jun;81(6):1395-403. (BU15, FA, FC)

Trowald-Wigh G, Johannisson A, Håkansson L. Canine neutrophil adhesion proteins and Fc-receptors in healthy dogs and dogs with adhesion protein deficiency, as studied by flow cytometry. Vet Immunol Immunopathol. 1993 Oct;38(3-4):297-310. (BU15, FC)

Postigo AA, Corbí AL, Sánchez-Madrid F, de Landázuri MO. Regulated expression and function of CD11c/CD18 integrin on human B lymphocytes. Relation between attachment to fibrinogen and triggering of proliferation through CD11c/CD18. J Exp Med. 1991 Dec 1;174(6):1313-22. (BU15, FC)