Human BAFF Instant ELISA

Description: The human BAFF Instant ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human BAFF in cell culture supernatants, human serum, plasma, or other body fluids. The human BAFF Instant ELISA is for research use only. Not for diagnostic or therapeutic procedures.

BAFF is a member of the TNF family, whose members induce pleiotropic biological responses including cell growth, differentiation and cell death. BAFF is expressed by T cells and dendritic cells.

BAFF was shown to mediate survival of peripheral immature B lymphocytes. Together with another member of the TNF superfamily, APRIL, BAFF is involved in the resistance to apoptosis of B-cell chronic lymphocytic leukaemia, B-CLL, through an autocrine pathway. BAFF turned out to be a key regulator of B-cell homeostasis.
Increased levels of BAFF in human sera were also found in primary bilary cirrhosis, autoimmune diabetes, rheumatic diseases, and Sjogren's syndrome.

Citations: Morimoto,S.; Nakano,S.; Watanabe,T.; Tamayama,Y.; Mitsuo,A.; Nakiri,Y.; Suzuki,J.; Nozawa,K.; Amano,H.; Tokano,Y.; Kobata,T.; Takasaki,Y.. Expression of B-cell activating factor of the tumour necrosis factor family (BAFF) in T cells in active systemic lupus erythematosus: the role of BAFF in T cell-dependent B cell pathogenic autoantibody production. Rheumatology 2007;46:1083-1086. (Link)

Thangarajh,M.; Masterman,T.; Helgeland,L.; Rot,U.; Jonsson,M.V.; Eide,G.E.; Pirskanen,R.; Hillert,J.; Jonsson,R.. The thymus is a source of B-cell-survival factors-APRIL and BAFF-in myasthenia gravis. Journal of Neuroimmunology 2006;178:161-166. (Link)

Breitkreutz,I.; Raab,M.; Vallet,S.; Hideshima,T.; Raje,N.; Mitsiades,C.; Chauhan,D.; Okawa,Y.; Munshi,N.; Richardson,P.; Anderson,K.. Lenalidomide inhibits osteoclastogenesis, survival factors and bone-remodeling markers in multiple myeloma. Leukemia 2008;22:1925-1932. (Link)

References: Kalled,S.L.. BAFF: a novel therapeutic target for autoimmunity. Curr.Opin.Investig.Drugs 2002;3:1005-1010. (Link)

Batten,M.; Groom,J.; Cachero,T.G.; Qian,F.; Schneider,P.; Tschopp,J.; Browning,J.L.; MacKay,F.. BAFF mediates survival of peripheral immature B lymphocytes 14. J.Exp.Med. 2000;192:1453-1466. (Link)

He,B.; Chadburn,A.; Jou,E.; Schattner,E.J.; Knowles,D.M.; Cerutti,A.. Lymphoma B cells evade apoptosis through the TNF family members BAFF/BLyS and APRIL. J.Immunol. 2004;172:3268-3279. (Link)

Roschke,V.; Sosnovtseva,S.; Ward,C.D.; Hong,J.S.; Smith,R.; Albert,V.; Stohl,W.; Baker,K.P.; Ullrich,S.; Nardelli,B.; Hilbert,D.M.; Migone,T.S.. BLyS and APRIL form biologically active heterotrimers that are expressed in patients with systemic immune-based rheumatic diseases. J.Immunol. 2002;169:4314-4321. (Link)

Kalled,S.L.; Ambrose,C.; Hsu,Y.M.. BAFF: B cell survival factor and emerging therapeutic target for autoimmune disorders. Expert.Opin.Ther.Targets. 2003;7:115-123. (Link)

MacKay,F.; Schneider,P.; Rennert,P.; Browning,J.. BAFF AND APRIL: a tutorial on B cell survival. Annu.Rev.Immunol. 2003;21:231-264. (Link)

Schneider,P.; MacKay,F.; Steiner,V.; Hofmann,K.; Bodmer,J.L.; Holler,N.; Ambrose,C.; Lawton,P.; Bixler,S.; cha-Orbea,H.; Valmori,D.; Romero,P.; Werner-Favre,C.; Zubler,R.H.; Browning,J.L.; Tschopp,J.. BAFF, a novel ligand of the tumor necrosis factor family, stimulates B cell growth. J.Exp.Med. 1999;189:1747-1756. (Link)

Schneider,P.; Tschopp,J.. BAFF and the regulation of B cell survival. Immunol.Lett. 2003;88:57-62. (Link)

MacKay,F.; Browning,J.L.. BAFF: a fundamental survival factor for B cells. Nat.Rev.Immunol. 2002;2:465-475. (Link)

Melchers,F.. Actions of BAFF in B cell maturation and its effects on the development of autoimmune disease. Ann.Rheum.Dis. 2003;62 Suppl 2:ii25-ii27. (Link)

Mackay,I.R.; Groom,J.; Mackay,C.R.. Levels of BAFF in serum in primary biliary cirrhosis and autoimmune diabetes. Autoimmunity 2002;35:551-553. (Link)

Mariette,X.; Roux,S.; Zhang,J.; Bengoufa,D.; Lavie,F.; Zhou,T.; Kimberly,R.. The level of BLyS (BAFF) correlates with the titre of autoantibodies in human Sjogren's syndrome. Ann.Rheum.Dis. 2003;62:168-171. (Link)

MacLennan,I.; Vinuesa,C.. Dendritic cells, BAFF, and APRIL: innate players in adaptive antibody responses. Immunity. 2002;17:235-238. (Link)

Kern,C.; Cornuel,J.F.; Billard,C.; Tang,R.; Rouillard,D.; Stenou,V.; Defrance,T.; jchenbaum-Cymbalista,F.; Simonin,P.Y.; Feldblum,S.; Kolb,J.P.. Involvement of BAFF and APRIL in the resistance to apoptosis of B-CLL through an autocrine pathway. Blood 2004;103:679-688. (Link)

Huard,B.; Arlettaz,L.; Ambrose,C.; Kindler,V.; Mauri,D.; Roosnek,E.; Tschopp,J.; Schneider,P.; French,L.E.. BAFF production by antigen-presenting cells provides T cell co-stimulation. Int.Immunol. 2004;16:467-475. (Link)

Avery,D.T.; Kalled,S.L.; Ellyard,J.I.; Ambrose,C.; Bixler,S.A.; Thien,M.; Brink,R.; MacKay,F.; Hodgkin,P.D.; Tangye,S.G.. BAFF selectively enhances the survival of plasmablasts generated from human memory B cells. J.Clin.Invest 2003;112:286-297. (Link)

MacKay,F.; Ambrose,C.. The TNF family members BAFF and APRIL: the growing complexity. Cytokine Growth Factor Rev. 2003;14:311-324. (Link)