Anti-Human CD95 (APO-1/Fas) Purified

Description: Immunohistochemistry:
APO-1-1 (BMS151, IgG1) is an isotype-switch variant of the original clone APO-1-3 (IgG3). Since undesired side reactions in immunohistochemistry are minimal for IgG1 antibodies, BMS151 is especially useful for immunohistology on cryostat sections and on cytospin preparations.

Flow cytometry:
BMS151 is also suitable as primary antibody in staining for FACS analysis, BMS151FI can be used for direct staining of cells, BMS151BT can be used in a two-step procedure using the biotin-(strept-)avidin system. Recommended concentration for the unlabelled antibody is 1 - 2 æg/ml and a 1:20 dilution of the labelled antibodies, as determined on SKW6.4 cells.

Citations: Rajasagi,Mohini; Vitacolonna,Mario; Benjak,Bojan; Marhaba,Rachid; Zoller,Margot. CD44 promotes progenitor homing into the thymus and T cell maturation. Journal of Leukocyte Biology 2009;85:251-261. (Link)

Fluhr,Herbert; Krenzer,Stefanie; Stein,Gerburg M.; Stork,Bjorn; Deperschmidt,Margarita; Wallwiener,Diethelm; Wesselborg,Sebastian; Zygmunt,Marek; Licht,Peter. Interferon-{gamma} and tumor necrosis factor-{alpha} sensitize primarily resistant human endometrial stromal cells to Fas-mediated apoptosis. Journal of Cell Science 2007;120:4126-4133. (Link)

Maeda,Hiroshi; Hori,Seiji; Nishitoh,Hideki; Ichijo,Hidenori; Ogawa,Osamu; Kakehi,Yoshiyuki; Kakizuka,Akira. Tumor Growth Inhibition by Arsenic Trioxide (As2O3) in the Orthotopic Metastasis Model of Androgen-independent Prostate Cancer. Cancer Research 2001;61:5432-5440. (Link)

Mahajan,Sandeep; Ghosh,Sutapa; Sudbeck,Elise A.; Zheng,Yaguo; Downs,Suzanne; Hupke,Michael; Uckun,Fatih M.. Rational Design and Synthesis of a Novel Anti-leukemic Agent Targeting Bruton's Tyrosine Kinase (BTK), LFM-A13 [alpha -Cyano-beta -Hydroxy-beta -Methyl-N-(2,5-Dibromophenyl)Propenamide]. Journal of Biological Chemistry 1999;274:9587-9599. (Link)

Vassilev,Alexei; Ozer,Zahide; Navara,Christopher; Mahajan,Sandeep; Uckun,Fatih M.. Bruton's Tyrosine Kinase as an Inhibitor of the Fas/CD95 Death-inducing Signaling Complex. Journal of Biological Chemistry 1999;274:1646-1656. (Link)