Anti-Human alpha beta TCR PE

Description: This WT31 monoclonal antibody recognizes the human αβ T cell receptor (TCR) complexed with the CD3ε chain. Composed of a 44-kDa α and a 37-kDa β chain, TCR specificity is typically determined by Vα, Jα, Vβ, Dβ, and Jβ gene rearrangement. The TCR is often associated with the CD3 complex, which is composed of at least five polypeptides. The TCR/CD3 complex recognizes and binds to major histocompatibility complex-bound antigen presented by antigen presenting cells such as B cells, dendritic cells, and macrophages. These events lead to T cell activation, resulting in cellular signaling and cytokine production.

The WT31 monoclonal antibody has been reported to induce proliferation of resting T cells, as well as inhibit cytotoxic T cell activity. In addition, some antibodies against CD3 partially inhibit CD3; clone SK7 is recommended for use in two-color experiments.

References: Salmerón A, Sánchez-Madrid F, Ursa MA, Fresno M, Alarcón B. A conformational epitope expressed upon association of CD3-epsilon with either CD3-delta or CD3-gamma is the main target for recognition by anti-CD3 monoclonal antibodies. J Immunol. 1991 Nov 1;147(9):3047-52. (WT31, FC)

Alarcon B, De Vries J, Pettey C, Boylston A, Yssel H, Terhorst C, Spits H. The T-cell receptor gamma chain-CD3 complex: implication in the cytotoxic activity of a CD3+ CD4- CD8- human natural killer clone. Proc Natl Acad Sci U S A. 1987 Jun;84(11):3861-5. (WT31, FC)

Spits H, Borst J, Tax W, Capel PJ, Terhorst C, de Vries JE. Characteristics of a monoclonal antibody (WT-31) that recognizes a common epitope on the human T cell receptor for antigen. J Immunol. 1985 Sep;135(3):1922-8. (WT31, FC)