Anti-Human alpha-Fetoprotein Alexa Fluor® 488

Also known as: AFP

Clone: AFP3

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 53-6583

Cat. No.	Size
53-6583-80	25 ug
53-6583-82	100 ug
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Immunocytochemistry of fixed and permeabilized HepG2 cells using 10 ug/ml of Mouse IgG1 Isotype...View More

Description

Description: This AFP3 monoclonal antibody reacts with human alpha-fetoprotein (AFP). This 70-kDa secretory protein is a member of the albumin gene family. Synthesized by the yolk sac and fetal liver during embryogenesis, AFP protein levels are highest in fetal serum. After birth, serum AFP levels decrease dramatically. In fact, AFP is nearly undetectable in normal adult serum. However, hepatocellular carcinoma and germ cell teratoblastoma, as well as liver regeneration, viral hepatitis, and cirrhosis, leads to elevated AFP serum levels in adults. As such, detection of this protein is frequently used as a diagnostic marker for these conditions.
When performing western blotting or immunohistochemistry on paraffin section, we recommend the use of monoclonal antibody 1E8 (cat. 14-9499).

Details

Host	Mouse
Isotype	IgG1, kappa
Reactivity	Human
Conjugate	Alexa Fluor 488
Laser	Blue Laser
Emit	519 nm
Excite	488 nm
Legal	Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Reported Applications	Immunocytochemistry

Documentation

TDS Link	Download TDS

Additional Formats

Cat. No.	Name	Excite	Emit	Application	Reg.
14-6583	Anti-Human alpha-Fetoprotein Purified			ICC, WB	RUO

Related Products

Cat. No.	Name	Excite	Emit	Application	Reg.
53-4714	Mouse IgG1 K Isotype Control Alexa Fluor® 488	488 nm	519 nm	FC, ICC, IHC	RUO

References

References: Lazarevich NL. Molecular mechanisms of alpha-fetoprotein gene expression. Biochemistry (Mosc). 2000 Jan;65(1):117-33. Review.

Kuo CY, Fu J, Yeh MY, Su SL, Lee CY. Generation of monoclonal antibodies to alpha-fetoprotein and application in solid-phase enzyme immunoassay. Biotechnol Appl Biochem. 1989 Feb;11(1):96-104. (AFP3, ELISA, Pubmed)

View More Products

Antigens	Area of Biology	Cell Type	Formats
AFP	Cell Cycle & Proliferation	Embryonic Stem Cells	Alexa Fluor 488