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Anti-Human ACT1 Purified

Also known as: Nuclear Factor kappa B Activator 1, TRAF3 interacting protein 2, TRAF3IP2, CIKS

Clone: 9ACT12

RUO: For Research Use Only. Not for use in diagnostic procedures.

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SKU# 14-4040

Cat. No. Size
14-4040-80 25 ug
14-4040-82 100 ug

Data for Anti-Human ACT1 Purified.

Immunoblotting of reduced human peripheral blood mononuclear cell lysates probed with 5 µg/mL of...View More

  • Data for Anti-Human ACT1 Purified.
Description

Description: The monoclonal antibody 9ACT12 reacts with human Act1 (NF-κB activator 1), also known as CIKS (a connection to IkB kinase and stress-activated kinase). Originally cloned for its ability to activate NF-κB and its direct interaction with IKKγ, Act1 has been subsequently shown to play several roles in immunity. In B cells, it is a negative regulator of CD40 and BAFFR signaling affecting reduced survival. This regulation is through direct interaction of Act1 with CD40 or BAFFR and TRAF3. In addition, Act1 is involved in IL-17R signaling. It interacts with IL-17RA through the homotypic SEFIR interaction domain and is critical for downstream NF-κB- and C/EBP-mediated inflammatory responses.

Details
Host Rat
Isotype IgG1
Reactivity Human
Conjugate Purified
Reported Applications Western Blotting
Documentation
TDS Link Download TDS
Related Products
Cat. No. Name Excite Emit Application Reg.
14-4301 Rat IgG1 K Isotype Control Purified FC, ICC, IHC RUO
References

References: Ryzhakov G, Blazek K, Lai CC, Udalova IA. IL-17 Receptor Adaptor Protein Act1/CIKS Plays an Evolutionarily Conserved Role in Antiviral Signaling. J Immunol. 2012 Nov 15;189(10):4852-8. doi: 10.4049/jimmunol.1200428.(WB, PubMed)

Shen F, Li N, Gade P, Kalvakolanu DV, Weibley T, Doble B, Woodgett JR, Wood TD, Gaffen SL. IL-17 receptor signaling inhibits C/EBPbeta by sequential phosphorylation of the regulatory 2 domain. Sci Signal. 2009 Feb 24;2(59):ra8.

Swaidani S, Bulek K, Kang Z, Liu C, Lu Y, Yin W, Aronica M, Li X. The critical role of epithelial-derived Act1 in IL-17- and IL-25-mediated pulmonary inflammation. J Immunol. 2009 Feb 1;182(3):1631-40.

Claudio E, Sønder SU, Saret S, Carvalho G, Ramalingam TR, Wynn TA, Chariot A, Garcia-Perganeda A, Leonardi A, Paun A, Chen A, Ren NY, Wang H, Siebenlist U. The adaptor protein CIKS/Act1 is essential for IL-25-mediated allergic airway inflammation. J Immunol. 2009 Feb 1;182(3):1617-30.

Qian Y, Giltiay N, Xiao J, Wang Y, Tian J, Han S, Scott M, Carter R, Jorgensen TN, Li X. Deficiency of Act1, a critical modulator of B cell function, leads to development of Sjögren's syndrome. Eur J Immunol. 2008 Aug;38(8):2219-28.


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