Anti-HIF-1 alpha Purified

Description: This ESEE122 monoclonal antibody reacts with hypoxia inducible factor-1 alpha. The hypoxia inducible factor (HIF-1) is a transcription factor composed of an inducible alpha form and the constitutively expressed beta form, also called the aryl hydrocarbon receptor nuclear translocator (ARNT). Both alpha and beta subunits of HIF-1 contain basic helix-loop-helix motifs and function to activate transcription of genes in response to reduced oxygen levels. Under normoxic conditions, HIF-1 alpha is rapidly degraded, while HIF-1 alpha expression is induced and degradation inhibited under hypoxic conditions. HIF-1 alpha can be found in the cytoplasm and/or in the nucleus under normoxic conditions, but translocates to the nucleus under hypoxic conditions. HIF-1 is upregulated in several cancer types and functions to control genes involved in angiogenesis, cell survival and T cell development. Recent studies have shown the importance of HIF-1 alpha in balancing Th17 and Treg development.

This ESEE122 antibody has also been reported to cross-react with bovine, rat, and mouse hypoxia inducible factor-1 alpha.

References: Dang EV, Barbi J, Yang HY, et al. Control of T(H)17/T(reg) balance by hypoxia-inducible factor 1. Cell. 2011 Sep 2;146(5):772-84.

Ibrahim NO, Hahn T, Franke C, Stiehl DP, Wirthner R, Wenger RH, Katschinski DM. Induction of the hypoxia-inducible factor system by low levels of heat shock protein 90 inhibitors. Cancer Res. 2005 Dec 1;65(23):11094-100.

Jung Y, Isaacs JS, Lee S, Trepel J, Liu ZG, Neckers L. Hypoxia-inducible factor induction by tumour necrosis factor in normoxic cells requires receptor-interacting protein-dependent nuclear factor kappa B activation. Biochem J. 2003 Mar 15;370(Pt 3):1011-7.