GSK3 beta (Total/Phospho) InstantOne™ ELISA

Also known as: Glycogen synthase kinase-3 beta

RUO: For Research Use Only. Not for use in diagnostic procedures.

Attention: The Total/Phospho InstantOne ELISAs contain reagents for a total of 96 wells (24 wells total/72 wells phospho). Please contact Technical Service for a lot specific COA.

SKU# 85-86173

Cat. No.	Size
85-86173-11	96 tests
Please inquire about custom configurations or bulk packaging.

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MCF7 cells were treated with either the PI3 Kinase inhibitor Wortmannin (10uM) (-) or insulin...View More

Description

Description: The InstantOne™ ELISA is specifically engineered for accurate measurement of total and phosphorylated human and mouse GSK3b (Ser9) in cell lysates. Detection of total and phosphorylated rat GSK3b (Ser9) is also expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.

Glycogen Synthase Kinase 3 (GSK3) is a Ser/Thr kinase that exists as two isoforms, GSK3a and GSK3b. Unlike other kinases, phosphorylation at Ser9 and Ser21 for GSK3a and GSK3b, respectively, leads to inactivation of this enzyme. GSK3 has been implicated in several diseases, including cancer and diabetes, through its phosphorylation of glycogen synthase. GSK3 has also been associated with neurodegenerative diseases such as Alzheimer's disease due to its phosphorylation of the neuronal Tau protein.

Details

Reactivity	Human
Suitable Sample Types	Tested on cell culture samples. Expected to work on many other sample types, but have not been tested at this time.
Components	The kit includes sufficient reagents to perform 24 wells of the total and 72 wells of the phospho-specific detection for a total of 96 wells. User Manual InstantOne™ Assay Plate (Part No. IOAP96) - 96 well (sealed) Cell Lysis Buffer (5X) (Part No. IOCLB1) - 8 mL Enhancer Solution (Part No. IOES1) - 0.5 mL Wash Buffer (10X) (Part No. IOWB1) - 8 mL GSK3b (Total) Capture Antibody Reagent (Part No. IOGSKCAR1M) - 1 x 0.75 mL phospho-GSK3b (Ser9) Capture Antibody Reagent (Part No. IOPGSKCAR1M) - 3 x 0.75 mL GSK3b (Total) Detection Antibody Reagent (Part No. IOGSKDAR1M ) - 1 x 0.75 mL phospho-GSK3b (Ser9) Detection Antibody Reagent (Part No. IOPGSKDAR1M) - 3 x 0.75 mL Positive Control Cell Lysate (lyophilized) (Part No. IOGSKPC1) - 1 ea Detection Reagent (TMB Substrate) (Part No. IODR1) - 10 mL Stop Solution (Part No. IOSS1) - 10 mL Plate Seal - 1 ea
Reported Applications	ELISA

Documentation

TDS Link	Download TDS
User Manual	Download User Manual

Related Products

Cat. No.	Name	Application	Reg.
IOCLB	InstantOne ELISA Cell Lysis Buffer (5X)	ELISA	RUO
IOES	InstantOne ELISA Enhancer Solution	ELISA	RUO
IOWB	InstantOne ELISA Wash Buffer(10X)	ELISA	RUO
85-86171	GSK3 beta (Total) InstantOne™ ELISA	ELISA	RUO
85-86172	phospho-GSK3 beta (Ser9) InstantOne™ ELISA	ELISA	RUO

References

View More Products

Antigens	Area of Biology
GSK	Cell Signaling Metabolism