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Foxp3 / Transcription Factor Staining Buffer Set

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 00-5523

Cat. No. Size
00-5523-00 Kit
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Data for Foxp3 Staining Buffer Set.

Description

Description: The Foxp3 Staining Buffer Set have been formulated and optimized for staining with the the Foxp3 antibodies, FJK-16s, NRRF-30, PCH101, 236A/E7 and eBio7979 monoclonal antibodies. It has also been shown to work for other transcription factors such as Nanog, Tbet, Gata-3, Ror gamma as well as cytokines.

Please see our Frequently Asked Questions regarding the usage of eBioscience Foxp3 antibodies and staining reagents.

Details
Components eBioscience Fixation/Permeabilization Concentrate (cat. 00-5123): 30 ml. Store at 4°C. Avoid agitation. Please use within expiration date indicated on the bottle. This is a 4X stock solution that must be diluted prior to use with the Fixation/Permeabilization Diluent. Dilute 1 part Concentrate with 3 parts Diluent. Caution: This solution contains formaldehyde, which is toxic and a suspected carcinogen. Contact with eyes, skin and mucous membranes should be avoided. Wear proper protective clothing and gloves.
eBioscience Fixation/Permeabilization Diluent (cat. 00-5223): 100 ml. Store at 4°C. The diluent is intented to be used in combination with the Fixation/Permeabilization Concentrate.
eBioscience Permeabilization Buffer (10X) (cat. 00-8333): 100 ml. Store at 4°C. Dilute to 1X with deionized/distilled water and store at 4°C. Caution: Harmful if swallowed or irritant by contact. Wear proper protective clothing and gloves. Note: The 10X Permeabilization Buffer has a natural tendency to precipitate, however, its function is not affected by this. To clarify, the solution can be filtered after dilution to 1X working solution.
Reported Applications Intracellular Staining Followed by Flow Cytometric Analysis
Documentation

For complete product information, please download the TDS or IFU document.

TDS Link Download TDS
Additional Formats
References

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Area of BiologyMiscellaneous
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