Foxp3 / Transcription Factor Staining Buffer Set

GPR: General Purpose Reagents. For Laboratory Use.

SKU# 00-5523

Cat. No.	Size
00-5523-00	Kit
Please inquire about custom configurations or bulk packaging.

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Description

Description: The Foxp3 Staining Buffer Set have been formulated and optimized for staining with the the Foxp3 antibodies, FJK-16s, NRRF-30, PCH101, 236A/E7 and eBio7979 monoclonal antibodies. It has also been shown to work for other transcription factors such as Nanog, Tbet, Gata-3, Ror gamma as well as cytokines.

Please see our Frequently Asked Questions regarding the usage of eBioscience Foxp3 antibodies and staining reagents.

Details

Components	eBioscience Fixation/Permeabilization Concentrate (cat. 00-5123): 30 ml. Store at 4°C. Avoid agitation. Please use within expiration date indicated on the bottle. This is a 4X stock solution that must be diluted prior to use with the Fixation/Permeabilization Diluent. Dilute 1 part Concentrate with 3 parts Diluent. Caution: This solution contains formaldehyde, which is toxic and a suspected carcinogen. Contact with eyes, skin and mucous membranes should be avoided. Wear proper protective clothing and gloves. eBioscience Fixation/Permeabilization Diluent (cat. 00-5223): 100 ml. Store at 4°C. The diluent is intented to be used in combination with the Fixation/Permeabilization Concentrate. eBioscience Permeabilization Buffer (10X) (cat. 00-8333): 100 ml. Store at 4°C. Dilute to 1X with deionized/distilled water and store at 4°C. Caution: Harmful if swallowed or irritant by contact. Wear proper protective clothing and gloves. Note: The 10X Permeabilization Buffer has a natural tendency to precipitate, however, its function is not affected by this. To clarify, the solution can be filtered after dilution to 1X working solution.
Reported Applications	Intracellular Staining Followed by Flow Cytometric Analysis

Documentation

TDS Link	Download TDS

Related Products

Cat. No.	Name	Excite	Emit	Application	Reg.
12-4776	Anti-Human Foxp3 PE	488 - 561 nm		FC	RUO
12-4875	Anti-Mouse EOMES PE	488 - 561 nm		FC	RUO
12-5773	Anti-Mouse/Rat Foxp3 PE	488 - 561 nm		FC	RUO
12-5825	Anti-Human/Mouse T-bet PE	488 - 561 nm	575 nm	FC	RUO
12-6988	Anti-Human/Mouse ROR gamma (t) PE	488 - 561 nm	575 nm	FC	RUO
12-9966	Anti-Human/Mouse Gata-3 PE	488 - 561 nm	575 nm	FC	RUO
53-5761	Anti-Mouse Nanog Alexa Fluor® 488	488 nm	519 nm	FC, ICC	RUO
12-5760	Anti-Mouse Bcl-6 PE	488 - 561 nm	575 nm	FC	RUO

References

View More Products

Area of Biology	Miscellaneous
Transcription Factors	Buffers and Solutions