Flow Cytometry Learning Center

Learn about flow cytometry methods and technologies

The purpose of this learning center is to connect scientists (whether new or experienced) to our many resources for learning about flow cytometry applications, techniques and basic principles by providing a few key points of entry into the vast content.

Flow Cytometry Guided Learning

Flow Cytometry Guided Learning

Find courses for basic applications and techniques used in flow cytometry

Molecular Probes School of Fluorescence

eLearning Courses

Flow Cytometry Subtopics

Flow cytometry guided learning

Designed for beginners, our guided learning experiences and eLearning courses were developed to present experimental and research topics in modular fashion, allowing users to tackle one topic at a time at their own pace. Choose a topic below and get started!

schematic of notepaper

Molecular Probes School of Fluorescence

The Molecular Probes School of Fluorescence was developed by our in-house bench scientists, featuring everything we wish we'd known when we first started working with fluorescent reagents, including the basics of fluorescence, imaging, and flow cytometry.

eLearning Courses

Our eLearning courses were developed to provide a succinct, contextual summary of common laboratory techniques and methods only used by researchers with an interest in protein and cell analysis workflows.


Flow cytometry subtopics

Areas of biology information

Flow cytometry is a powerful tool in the study of many areas of biology. In this subtopic section you will find educational assets for help with immunology, immuno-oncology, cancer, stem cell research and more.

Cell isolation, expansion and differentiation information

Cell isolation, expansion and differentiation are critical processes and techniques for studying cell biology. Here you will find educational resources including videos, application notes and articles to help you better understand these processes.

Controls, compensation and calibration information

Controls, compensation and calibration are all critical to obtaining accurate results in flow cytometric analysis. In this section you will find educational resources to help you set up your flow cytometer and analyze your samples.

Flow cytometry assays information

Learn about assays and techniques for detecting and monitoring cell health and function by flow cytometry. Topics include apoptosis, cell counting, proliferation and viability as well as cell cycle, endocytosis, oxidative stress and more.

Flow cytometry instrumentation information

These resources will help you increase your understanding of the fundamentals and scientific principles of flow cytometry, and expand your abilities for specialty applications and techniques.

We provide free flow cytometry panel design service as well as free panel builder webtool

FREE: Flow Cytometry Panel Design Service and Panel Builder Tool

For a personalized panel design service, contact our flow cytometry panel design team. Our flow cytometry scientists work with you to develop optimized panels (conventional and spectral) to fit your experiment and your instrument. You can use our panel design service as many times as you need—free of charge, and all panels are reviewed by at least 2 experts.

We also offer a free, do-it-yourself (DIY) webtool—Flow Cytometry Panel Builder Tool. This online tool guides you through flow cytometry panel design, providing a simplified, customizable experience to fit your flow cytometry panel design needs.


For Research Use Only. Not for use in diagnostic procedures.