eFluor® 605 Nanocrystal Conjugation Kit – Amine Reactive (To be discontinued)

GPR: General Purpose Reagents. For Laboratory Use.

Attention: To be discontinued March 31st 2013

SKU# 88-7071

Cat. No.	Size
88-7071-98	Kit
88-7071-00	Kit
Please inquire about custom configurations or bulk packaging.

To enable ordering, please select your country. Cookies will need to be enabled to continue on our website. View our Privacy Policy.

Flow cytometry and immunocytochemistry using antibodies conjugated with the eFluor® 605 Nanocrystal...View More

Description

Description: The eFluor® 605 Nanocrystal Conjugation Kit – Amine Reactive provides all of the necessary components for 2 separate conjugations and purifications of 200 µg of antibody to eFluor® 605 nanocrystals. This kit utilizes any available primary amine on the antibody for cross-linking to a specially-modified PEG molecule on the outer surface of the nanocrystal. Although the kit has been optimized for antibodies, water-soluble molecules with a primary amine group and a molecular weight from 30 kDa to 150 kDa may potentially be conjugated to eFluor® 605 nanocrystals using this kit.

A simple protocol with diagrams is provided with the kit to help ensure successful conjugation. Additional information and Frequently Asked Questions can be found in the Technical Support section.

Details

Components	Concentrator, 30 kDa MWCO (I.A). 2 units Dimethylformamide (DMF) (II.A). 1 vial containing 100 uL; Handle with gloves and dispose of properly Amine NC Kit Activator (II.B). 2 vials Amine NC Kit Activation Buffer (II.C). 1 vial containing 1 mL Amine NC Kit Conjugation Buffer (III.A). 2 vials containing 1 mL each Spin Column (25 kDa) (III.B). 2 columns Amine NC Kit eFluor® 605 Nanocrystals (IV.A). 2 vials Amine NC Kit Catalyst (IV.B). 2 vials containing 100 uL each Amine NC Kit Quencher (V.A). 2 vials NC Kit Purification Buffer (VI.A). 1 tube containing 14 mL Concentrator, 100 kDa MWCO (VI.B). 2 units
Legal	Under patent number: US 7,939,170 and additional pending patent application(s)
Reported Applications	Flow Cytometric Analysis, Immunohistochemical Staining of Frozen Tissue Sections, Immunohistochemical Staining of Formalin-Fixed Paraffin Embedded Tissue Sections, Immunocytochemistry, Biomolecule Conjugation

Documentation

TDS Link	Download TDS

Related Products

Cat. No.	Name	Excite	Emit	Application	Reg.
00-4952	IHC/ICC Blocking Buffer - High Protein			ICC, IHC	GPR
00-4958	Fluoromount-G™			ICC, IHC	GPR
14-0041	Anti-Mouse CD4 Purified			FC, IHC, IP	RUO
14-4502	Anti-alpha Tubulin Purified			IHC, IP, WB	RUO
50-0081	Anti-Mouse CD8a eFluor® 660 (Alexa Fluor® 647 Replacement)	633 - 647 nm	668 nm	FC, IHC	RUO

References