ES/iPS Cells
Ectodermal Layer
Endodermal Layer
Mesodermal Layer
Hematopoietic Stem Cells
Cancer Stem Cells
Recombinant Proteins for Stem Cell Culture & Differentiation

NEW Stem Cell Reagents

Anti-human CD34

Staining of normal human peripheral blood mononuclear cells with FITC anti-human CD45 (HI30) (cat. 11-0459) and Alexa Fluor® 647 Mouse IgG1, K Iso Cntrl (cat. 51-4714) (left) or Alexa Fluor® 647 anti-human CD34 (4H11) (right). Cells in the lymphocyte gate were used for analysis.

Anti-human CD133


Staining of normal human peripheral blood mononuclear cells with FITC anti-human CD45 (HI30) (cat. 11-0459) and PE Mouse IgG2b Iso Cntrl (cat. 12-4732) (left) or PE anti-human CD133 / AC133 (EMK08) (right). Cells in the lymphocyte gate were used for analysis.		293T (lanes 1 and 3) or human CD133-transduced 293T (lanes 2 and 4) cell lysates either non-reduced (lanes 1 and 2) or reduced (lanes 3 and 4) were loaded at 1X10⁵ cells/lane, probed with 2 ug/ml purified EMK08, and revealed with HRP anti-mouse IgG.

Anti-mouse Nanog

Quantitative Assessment of Embryonic Stem (ES) Cell Differentiation by Flow Cytometry

Down Regulation of Nanog in Differentiated Cells:
Flow cytometric analysis of Nanog in either untreated (left panel) or treated (right panel) F9 cells . Cells were cultured for three days with All-Trans-Retinoic Acid and then stained with either Alexa Fluor® 647 isotype control (Cat. No. 51-4321, open histogram) or Alexa Fluor® 647 Anti-Mouse Nanog (Cat. No. 51-5761, shaded histogram) using the Foxp3 Buffer Set (Cat. No. 00-5523).

Table 1: **ES/iPS Cell Markers**
ES/iPS Cell Markers
Specificity	Human	Mouse
β-catenin	2567	2567
CD9	0098	0091
CD15	0159	--
CD24	0247	0242
CD29 (Integrin β1)	0299	0291
CD30	--	0301
CD49f (Integrin a6)	0495	0495
CD338 (ABCG2)	8888	--
c-Kit (CD117)	1179	1171
c-Myc	6785	6785
Dll1 (Delta-like 1)	--	5767
E-Cadherin (CD324)	3249	3249
Eomes	4877	4875
EpCAM (TROP-1)	9326	5791
Jagged-2	--	3392
Nanog	5769	5761
Notch1	5785	5785
Notch3	--	5763
Notch4	--	5764
Oct 3/4	5841	5841
SSEA-1	8813	8813
SSEA-3	8833	8833
SSEA-4	8843	--
STAT3	6727	6727
TRA-1-60	8863	--
TRA-1-81	8883	--

Table 2: **Ectoderm Markers**
Ectoderm Markers
Specificity	Human	Mouse
Nestin	9843	5843
Notch1	5785	5785
Neural Markers
CD24 (HSA)	0247	0242
CD56 (NCAM)	--	0569
CD81 (TAPA-1)	--	0811
CD90 (Thy-1)	0909	0900
CD166 (ALCAM)	--	1661
CD325 (N-Cadherin)	3259	--
Nestin	9843	5843
Oligodendrocyte Markers
CD44	0441	0441
CD140a (PDGFRa)	--	1401
Neural Crest Markers
CD49d	0499	0492
CD57	0577	--
LPAM-1	--	5887
Notch1	5785	5785
Neural Stem Cell Markers
CD15	0159	--
CD24 (HSA)	0247	0242
CD29 (Integrin β1}	0299	0291
CD49f (Integrin α6)	0495	0495
CD54 (ICAM-1)	0549	0541
CD81	--	0811
CD95 (FAS/APO-1)	0959	0951
CD133	1338	1331
CD140a (PDGFRa)	--	1401
CD146	1469	--
CD184 (CXCR4)	9999	9991
CD338 (ABCG2)	8888	--
Nestin	9843	5843
Notch1	5785	5785
SSEA-1	8813	8813
Skin Precursor Markers
CD49d	0499	0492
CD166 (ALCAM)	--	1661
Foxj1	9965	9965
LPAM-1	--	5887
Nestin	9843	5843
Sca-1 (Ly-6A/E)	--	5981

Table 3: **Endoderm Markers**
Endoderm Markers
Specificity	Human	Mouse
β-Catenin	2567	2567
CD29 (Integrin β1)	0299	0291
CD31 (PECAM-1)	0319	0311
CD49f (Integrin α6)	0495	0495
CD90 (Thy-1)	0909	0900
CD184 (CXCR4)	9999	9991
c-Met (HGFR)	8858	8854
GATA-4	9980	9980
N-Cadherin (CD325)	3259	--
Sca-1 (Ly-6A/E)	--	5981

Mesoderm Markers
Specificity	Human	Mouse
Endothelial Cell Markers
CD14	0149	0141
CD15	0159	--
CD18 (Integrin β2)	0187	0181
CD29 (Integrin β1)	0299	0291
CD31 (PECAM-1)	0319	0311
CD34 (Mucosialin)	0349	0341
CD36	0369	0361
CD45 (LCA)	0459	0451
CD45R (B220)	0452	0452
CD49d (Integrin α4)	0499	0492
CD49e (Integrin α5)	0496	0493
CD49f (Integrin α6)	0495	0495
CD54 (ICAM-1)	0549	0541
CD56 (NCAM)	0568	--
CD62E (E-selectin)	0627	--
CD62L (L-selectin)	0629	0621
CD62P (P-selectin)	0628	0626
CD93 (C1qRp)	0939	5892
CD102	1029	1021
CD105 (Endoglin)	1057	1051
CD106 (VCAM-1)	1069	1061
CD109	1099	--
CD116 (GM-CSFR)	1169	--
CD117 (c-Kit)	1179	1171
CD120a (TNFR Type I)	--	1202
CD120b (TNFR Type II)	--	1203
CD124 (IL-4Rα)	1249	--
CD144 (VE-Cadherin)	1449	1441
CD146 (MCAM)	1469	--
CD147 (Neurothelin)	1472	1471
CD152 (CTLA-4)	1529	1522
CD157	1579	--
CD166 (ALCAM)	--	1661
CD184 (CXCR4)	9999	9991
CD201 (EPCR)	--	2012
CD209 (DC-SIGN)	2099	2092
CD252 (OX-40L)	--	5905
CD253 (TRAIL)	9927	5951
CD262 (TRAIL-R2)	9908	5883
CD309 (FLK-1, VEGF-R2))	--	5821
CD325 (N-cadherin)	3259	--
Nestin	9843	5843
Sca-1 (Ly-6A/E)	--	5981
STAT3	6727	6727
Cardiogenesis Markers
CD31	0319	0311
CD34	0349	0341
CD66a	--	0661
CD106	1069	1061
CD117 (c-Kit)	1179	1171
CD144 (VE-Cadherin)	1449	1441
CD166 (ALCAM)	--	1661
CD309 (FLK-1, VEGF-R2	--	5821
CD325 (N-Cadherin)	3259	--
Gata-4	9980	9980
Bone Marrow Precursor Markers (positive)
CD10	0106	--
CD13	0138	--
CD14	0149	0141
CD15	0159	--
CD29 (Integrin β1)	0299	0291
CD49d (Integrin α4)	0492	0499
CD49e (Integrin α5)	0496	0493
CD51 (Integrin αV)	--	0512
CD54 (ICAM-1)	0541	0549
CD71 (Transferrin Receptor)	0719	0711
CD73 (Ecto-5'-Nucleotidase)	--	0731
CD90 (Thy-1)	0909	0900
CD105 (Endoglin)	1057	1051
CD106 (VCAM-1)	1069	1061
CD117 (c-Kit)	1179	1171
CD120a (TNFR Type I)	--	1202
CD120b (TNFR Type II)	--	1203
CD166 (ALCAM)	--	1661
CD309 (Flk-1, VEGF-R2)	--	5821
Sca-1 (Ly-6A/E)	--	5981
Bone Marrow Precursor Markers (negative)
CD11a	0119	0111
CD11b	0118	0112
CD31 (PECAM-1)	0319	0311
CD34 (Mucosialin)	0349	0341
CD45 (LCA)	0459	0451
Adipose Precursor Markers (positive)
CD9	0098	0091
CD10	0106	--
CD13	0138	--
CD29 (Integrin β1)	0299	0291
CD44	0441	0441
CD49d (Integrin α4)	0499	0492
CD49e (Integrin α5)	0496	0493
CD54 (ICAM-1)	0549	0541
CD55	0559	--
CD73 (Ecto-5'-Nucleotidase)	--	0731
CD90 (Thy-1)	0909	0900
CD105 (Endoglin)	1057	1051
CD106 (VCAM-1)	1069	1061
CD146 (MCAM)	1469	--
CD166 (ALCAM)	--	1661
Adipose Precursor Markers (negative)
CD11b	0113	0112
CD14	0149	0141
CD19	0198	0199
CD34 (Mucosialin)	0349	0341
CD45 (LCA)	0459	0451
CD79a	--	0791
HLA-DR	9956	--

Table 4: **Hematopoietic Markers**
Hematopoietic Markers
Specificity	Human	Mouse
Lymphoid Markers
CD10	0106	--
CD34 (Mucosialin)	0349	0341
CD38	0388	0381
CD90 (Thy-1)	0909	0901
CD117 (c-Kit)	1179	1171
CD124 (IL-4Ra)	1249	--
CD127 (IL-7Ra)	1278	1271
HLA-DR	9952	--
Megakaryoblast Markers
CD34 (Mucosialin)	0349	0341
CD36	0369	0361
CD41	0418	0411
CD61 (Integrin β3)	0519	0611
HLA-DR	9952	--
Monoblast Markers
CD11c	0116	0114
CD13	0138	--
CD15	0159	--
CD33	0038	--
CD115 (FMS)	1159	1152
CD116 (GM-CSFR)	1169	--
Myeloblast Markers
CD13	0138	--
CD15	0159	--
CD33	0038	--
CD116 (GM-CSFR)	1169	--
Pro-Erythroblast Markers
CD71 (Transferrin Receptor)	0719	0711
CD105 (Endoglin)	1057	1051
TER-119	--	5921
HSC Markers (negative)
CD2	0029	0021
CD3	0039	0031
CD4	0049	0041
CD10	0106	--
CD13	-138	--
CD14	0149	0141
CD15	0159	--
CD16	0168	0161
CD19	0198	0191
CD20	0209	0201
CD24	0247	0242
CD33	0338	--
CD36	0369	0361
CD38	0388	0381
CD45 (LCA)	0459	0451
CD48	0489	0451
CD56 (NCAM)	0568	--
CD138 (Sydecan-1)	1389	--
CD244	2449	2441
GATA-3	9966	9966
HSC Markers (positive)
CD31 (PECAM-1)	0319	0311
CD34 (Mucosialin)	0349	0341
CD44	0441	0441
CD84	0849	--
CD90 (Thy-1)	0909	0901
CD90.1 (Thy-1.1)	--	0900
CD93 (C1qRp)	0938	5892
CD105 (Endoglin)	1057	1051
CD106 (VCAM-1)	1069	1061
CD117 (c-Kit)	1179	1171
CD201 (EPCR)	--	2012
CD150 (SLAM)	1509	1502
CD184 (CXCR4)	9999	9991
CD325 (N-Cadherin)	3259
CD338 (ABCG2)	8888	--
Flk-1 (VEGF-R2)	--	5821
Notch1	5875	5875
Sca-1 (Ly-6A/E)	--	5981
WASP	6987	6987
Hemangioblast Markers
CD31 (PECAM-1)	0319	0311
CD34 (Mucosialin)	0349	0341
CD144 (VE-Cadherin)	1449	1441
CD202 (Tie-2)	--	5987
CD309 (Flk-1, VEGF-R2)	--	5821
CD324 (E-Cadherin)	3249	3249

Table 5: **Cancer Stem Cell Markers**
Cancer Stem Cell Markers
Specificity	Human	Mouse
CD7	0079	--
CD10	0106	--
CD18 (Integrin β2)	0187	0181
CD19	0199	0193
CD20	0209	0201
CD24 (HSA)	0247	0242
CD27	0279	0271
CD29 (Integrin β1)	0299	0291
CD31 (PECAM-1)	0319	0311
CD33	0338	--
CD34 (Mucosialin)	0349	0341
CD38	0389	0381
CD44	0441	0441
CD49b (Integrin α2)	0498	0491
CD49f (Integrin α6)	0495	0495
CD74	0749	--
CD90 (Thy-1)	0909	0900
CD96 (Tactile)	0969	--
CD105 (Endoglin)	1057	1051
CD117 (c-Kit)	1179	1171
CD123 (IL-3Rα)	1239	1231
CD133 (Prominin-1)	1338	1331
CD138 (Syndecan-1)	1389	--
CD166 (ALCAM)	--	1661
CD184 (CXCR4)	9999	9991
CD324 (E-cadherin)	3249	3249
CD338 (ABCG2)	8888	--
Dll1	--	5767
EpCAM (TROP-1)	9326	5791
Jagged-2	--	3392
Nestin	9843	5843
Notch1	5785	5785
Notch3	--	5763
Notch4	--	5764
Podoplanin	9381	5381
SSEA-1	8813	8813
SSEA-3	8833	8833
SSEA-4	8843	--
TRA-1-60	8863	--
TRA-1-80	8883	--

Recombinant Proteins for Stem Cell Culture & Differentiation
Protein	Human	Mouse
ES/iPS Cell Culture
BMP2	8507	--
EGF	8988	8984
FGF-acidic	8987	8981
FGF-basic	8986	8982
Leukemia Inhibitory Factor (LIF)	--	8521
PDGF-AA	--	8989
PDGF-BB	8501	--
TGFβ	8348	--
TGFβ2	8368	--
Ectoderm Cell Culture
BMP2	8507	--
EGF	8988
GDNF	8506	--
IGF-1	--	8508
IGF-2	8505	--
NGF-b	--	8504
PDGF-AA	--	8989
PDGF-BB	8501	--
TGFβ	8348	--
TGFβ2	8368	--
Endoderm Cell Culture
BMP2	8507	--
EGF	8988	8984
HGF	8439	8431
IGF-1	--	8508
IGF-2	8505	--
IL-6	7066	7064
PDGF-AA	--	8989
PDGF-BB	8501	--
SCF	8349	8341
TGFβ	8348	--
TGFβ2	8368	--
VEGF	8359	8351
Mesoderm Cell Culture
BMP2	8507	--
CTGF	8503	--
G-CSF	8979	8971
GM-CSF	8339	8331
HGF	8439	8431
IGF-1	--	8508
IGF-2	8505	--
IL-6	8069	8061
Leptin	8509	8510
M-CSF	8985	8983
PDGF-AA	--	8989
PDGF-BB	8501	--
SCF	8349	8341
TGFβ	8348	--
TGFβ2	8368	--
VEGF	8359	8351
HSC Cell Culture
BMP2	8507	--
Flt-3L	8513	--
G-CSF	8979	8971
GM-CSF	8339	8331
HGF	8439	8431
IGF-1	--	8508
IGF-2	8505	--
IL-3	8039	8031
IL-6	8069	8061
IL-7	8079	8071
M-CSF	8985	8983
SCF	8349	8341
TGFβ	8348	--
TGFβ2	8648	--
TNFα	8329	8321
TSLP	8497	8498
VEGF	8359	8351