ES/iPS Cell Products
Ectodermal Layer Products
Endodermal Layer Products
Mesodermal Layer Products
Hematopoietic Stem Cell Products
Cancer Stem Cell Products
Recombinant Proteins for Stem Cell Culture & Differentiation

NEW Stem Cell Markers

Human Stem Cell Markers
Mouse Stem Cell Markers

Anti-human CD34

Staining of normal human peripheral blood mononuclear cells with FITC anti-human CD45 (HI30) (cat. 11-0459) and Alexa Fluor® 647 Mouse IgG1, K Iso Cntrl (cat. 51-4714) (left) or Alexa Fluor® 647 anti-human CD34 (4H11) (right). Cells in the lymphocyte gate were used for analysis.

Anti-human CD133


Staining of normal human peripheral blood mononuclear cells with FITC anti-human CD45 (HI30) (cat. 11-0459) and PE Mouse IgG2b Iso Cntrl (cat. 12-4732) (left) or PE anti-human CD133 / AC133 (EMK08) (right). Cells in the lymphocyte gate were used for analysis.		293T (lanes 1 and 3) or human CD133-transduced 293T (lanes 2 and 4) cell lysates either non-reduced (lanes 1 and 2) or reduced (lanes 3 and 4) were loaded at 1X10⁵ cells/lane, probed with 2 ug/ml purified EMK08, and revealed with HRP anti-mouse IgG.

Anti-mouse Nanog

Quantitative Assessment of Embryonic Stem (ES) Cell Differentiation by Flow Cytometry

Down Regulation of Nanog in Differentiated Cells:
Flow cytometric analysis of Nanog in either untreated (left panel) or treated (right panel) F9 cells . Cells were cultured for three days with All-Trans-Retinoic Acid and then stained with either Alexa Fluor® 647 isotype control (Cat. No. 51-4321, open histogram) or Alexa Fluor® 647 Anti-Mouse Nanog (Cat. No. 51-5761, shaded histogram) using the Foxp3 Buffer Set (Cat. No. 00-5523).

Table 1: **ES/iPS Cell Markers**
ES/iPS Cell Markers
Specificity	Human	Mouse
β-catenin	2567	2567
CD9	0098	0091
CD15	0159	--
CD24	0247	0242
CD29 (Integrin β1)	0299	0291
CD30	--	0301
CD49f (Integrin a6)	0495	0495
CD338 (ABCG2)	8888	--
c-Kit (CD117)	1179	1171
c-Myc	6785	6785
Dll1 (Delta-like 1)	--	5767
E-Cadherin (CD324)	3249	3249
Eomes	4877	4875
EpCAM (TROP-1)	9326	5791
Jagged-2	--	3392
Nanog	5769	5761
Notch1	5785	5785
Notch3	--	5763
Notch4	--	5764
Oct 3/4	5841	5841
SSEA-1	8813	8813
SSEA-3	8833	8833
SSEA-4	8843	--
STAT3	6727	6727
TRA-1-60	8863	--
TRA-1-81	8883	--

Table 2: **Ectoderm Markers**
Ectoderm Markers
Specificity	Human	Mouse
Nestin	9843	5843
Notch1	5785	5785
Neural Markers
CD24 (HSA)	0247	0242
CD56 (NCAM)	--	0569
CD81 (TAPA-1)	--	0811
CD90 (Thy-1)	0909	0900
CD166 (ALCAM)	--	1661
CD325 (N-Cadherin)	3259	--
Nestin	9843	5843
Oligodendrocyte Markers
CD44	0441	0441
CD140a (PDGFRa)	--	1401
Neural Crest Markers
CD49d	0499	0492
CD57	0577	--
LPAM-1	--	5887
Notch1	5785	5785
Neural Stem Cell Markers
CD15	0159	--
CD24 (HSA)	0247	0242
CD29 (Integrin β1}	0299	0291
CD49f (Integrin α6)	0495	0495
CD54 (ICAM-1)	0549	0541
CD81	--	0811
CD95 (FAS/APO-1)	0959	0951
CD133	1338	1331
CD140a (PDGFRa)	--	1401
CD146	1469	--
CD184 (CXCR4)	9999	9991
CD338 (ABCG2)	8888	--
Nestin	9843	5843
Notch1	5785	5785
SSEA-1	8813	8813
Skin Precursor Markers
CD49d	0499	0492
CD166 (ALCAM)	--	1661
Foxj1	9965	9965
LPAM-1	--	5887
Nestin	9843	5843
Sca-1 (Ly-6A/E)	--	5981

Table 3: **Endoderm Markers**
Endoderm Markers
Specificity	Human	Mouse
β-Catenin	2567	2567
CD29 (Integrin β1)	0299	0291
CD31 (PECAM-1)	0319	0311
CD49f (Integrin α6)	0495	0495
CD90 (Thy-1)	0909	0900
CD184 (CXCR4)	9999	9991
c-Met (HGFR)	8858	8854
GATA-4	9980	9980
N-Cadherin (CD325)	3259	--
Sca-1 (Ly-6A/E)	--	5981

Table 4: **Early Mesoderm Markers**
Early Mesoderm Markers
Specificity	Human	Mouse
CD31 (PECAM1)	0319	0311
CD34 (Mucosialin)	0349	0341
CD325 (N-Cadherin)	3259	--
Sca-1 (Ly-6A/E)	--	5981

Table 5: **Hematopoietic Markers**
Hematopoietic Markers
Specificity	Human	Mouse
Lymphoid Markers
CD10	0106	--
CD34 (Mucosialin)	0349	0341
CD38	0388	0381
CD90 (Thy-1)	0909	0901
CD117 (c-Kit)	1179	1171
CD124 (IL-4Ra)	1249	--
CD127 (IL-7Ra)	1278	1271
HLA-DR	9952	--
Megakaryoblast Markers
CD34 (Mucosialin)	0349	0341
CD36	0369	0361
CD41	0418	0411
CD61 (Integrin β3)	0519	0611
HLA-DR	9952	--
Monoblast Markers
CD11c	0116	0114
CD13	0138	--
CD15	0159	--
CD33	0038	--
CD115 (FMS)	1159	1152
CD116 (GM-CSFR)	1169	--
Myeloblast Markers
CD13	0138	--
CD15	0159	--
CD33	0038	--
CD116 (GM-CSFR)	1169	--
Pro-Erythroblast Markers
CD71 (Transferrin Receptor)	0719	0711
CD105 (Endoglin)	1057	1051
TER-119	--	5921
HSC Markers (negative)
CD2	0029	0021
CD3	0039	0031
CD4	0049	0041
CD10	0106	--
CD13	-138	--
CD14	0149	0141
CD15	0159	--
CD16	0168	0161
CD19	0198	0191
CD20	0209	0201
CD24	0247	0242
CD33	0338	--
CD36	0369	0361
CD38	0388	0381
CD45 (LCA)	0459	0451
CD48	0489	0451
CD56 (NCAM)	0568	--
CD138 (Sydecan-1)	1389	--
CD244	2449	2441
GATA-3	9966	9966
HSC Markers (positive)
CD31 (PECAM-1)	0319	0311
CD34 (Mucosialin)	0349	0341
CD44	0441	0441
CD84	0849	--
CD90 (Thy-1)	0909	0901
CD90.1 (Thy-1.1)	--	0900
CD93 (C1qRp)	0938	5892
CD105 (Endoglin)	1057	1051
CD106 (VCAM-1)	1069	1061
CD117 (c-Kit)	1179	1171
CD201 (EPCR)	--	2012
CD150 (SLAM)	1509	1502
CD184 (CXCR4)	9999	9991
CD325 (N-Cadherin)	3259
CD338 (ABCG2)	8888	--
Flk-1 (VEGF-R2)	--	5821
Notch1	5875	5875
Sca-1 (Ly-6A/E)	--	5981
WASP	6987	6987
Hemangioblast Markers
CD31 (PECAM-1)	0319	0311
CD34 (Mucosialin)	0349	0341
CD144 (VE-Cadherin)	1449	1441
CD202 (Tie-2)	--	5987
CD309 (Flk-1, VEGF-R2)	--	5821
CD324 (E-Cadherin)	3249	3249

Table 6: **Cancer Stem Cell Markers**
Cancer Stem Cell Markers
Specificity	Human	Mouse
CD7	0079	--
CD10	0106	--
CD18 (Integrin β2)	0187	0181
CD19	0199	0193
CD20	0209	0201
CD24 (HSA)	0247	0242
CD27	0279	0271
CD29 (Integrin β1)	0299	0291
CD31 (PECAM-1)	0319	0311
CD33	0338	--
CD34 (Mucosialin)	0349	0341
CD38	0389	0381
CD44	0441	0441
CD49b (Integrin α2)	0498	0491
CD49f (Integrin α6)	0495	0495
CD74	0749	--
CD90 (Thy-1)	0909	0900
CD96 (Tactile)	0969	--
CD105 (Endoglin)	1057	1051
CD117 (c-Kit)	1179	1171
CD123 (IL-3Rα)	1239	1231
CD133 (Prominin-1)	1338	1331
CD138 (Syndecan-1)	1389	--
CD166 (ALCAM)	--	1661
CD184 (CXCR4)	9999	9991
CD324 (E-cadherin)	3249	3249
CD338 (ABCG2)	8888	--
Dll1	--	5767
EpCAM (TROP-1)	9326	5791
Jagged-2	--	3392
Nestin	9843	5843
Notch1	5785	5785
Notch3	--	5763
Notch4	--	5764
Podoplanin	9381	5381
SSEA-1	8813	8813
SSEA-3	8833	8833
SSEA-4	8843	--
TRA-1-60	8863	--
TRA-1-80	8883	--

Table 7: Recombinant Proteins for Stem Cell Culture & Differentiation
Recombinant Proteins for Stem Cell Culture & Differentiation
Protein	Human	Mouse
ES/iPS Cell Culture
BMP2	8507	--
EGF	8988	8984
FGF-acidic	8987	8981
FGF-basic	8986	8982
PDGF-AA	--	8989
PDGF-BB	8501	--
TGFβ	8348	--
TGFβ2	8368	--
Ectoderm Cell Culture
BMP2	8507	--
EGF	8988
GDNF	8506	--
IGF-1	--	8508
IGF-2	8505	--
NGF-b	--	8504
PDGF-AA	--	8989
PDGF-BB	8501	--
TGFβ	8348	--
TGFβ2	8368	--
Endoderm Cell Culture
BMP2	8507	--
EGF	8988	8984
HGF	8439	8431
IGF-1	--	8508
IGF-2	8505	--
IL-6	7066	7064
PDGF-AA	--	8989
PDGF-BB	8501	--
SCF	8349	8341
TGFβ	8348	--
TGFβ2	8368	--
VEGF	8359	8351
Mesoderm Cell Culture
BMP2	8507	--
CTGF	8503	--
G-CSF	8979	8971
GM-CSF	8339	8331
HGF	8439	8431
IGF-1	--	8508
IGF-2	8505	--
IL-6	8069	8061
Leptin	8509	8510
M-CSF	8985	8983
PDGF-AA	--	8989
PDGF-BB	8501	--
SCF	8349	8341
TGFβ	8348	--
TGFβ2	8368	--
VEGF	8359	8351
HSC Cell Culture
BMP2	8507	--
Flt-3L	8513	--
G-CSF	8979	8971
GM-CSF	8339	8331
HGF	8439	8431
IGF-1	--	8508
IGF-2	8505	--
IL-3	8039	8031
IL-6	8069	8061
IL-7	8079	8071
M-CSF	8985	8983
SCF	8349	8341
TGFβ	8348	--
TGFβ2	8648	--
TNFα	8329	8321
TSLP	8497	8498
VEGF	8359	8351