eBioscience now offering over 60 PerCP-Cy5.5 Reagents!

Benefits of eBioscience PerCP-Cy5.5 tandems
eBioscience PerCP-Cy5.5 conjugates perform!
PerCP-Cy5.5 specificities
Supporting information: Filter recommendations & about PerCP

The benefits of eBioscience PerCP-Cy5.5 products make them a clear choice for use in FL3!

Features	Benefits
• Unique spectral properties of PerCP have limited contribution to FL2 (max 575nm) emission	• Enables easier multi-color cytometry! o Compatible with 4-colors of 488nm laser equipped systems o Enables robust 5 color cytometry off 1 laser for multi-laser systems such as the BD^TM LSRII using FITC, PE, PE-Cy5, PerCP-Cy5.5, PE-Cy7
• Cy5.5 modification of PerCP extends the effective Stokes shift of the fluorophore by >15nm via Fluorescence Resonance Energy Transfer	• Saves time on instrument set up because it requires only minimal FL3-%FL-2 compensation • Reduces the amount of 633nm excitation and compensation required off the 633nm laser on commonly used 2 laser systems
• Cy5.5 modification enhances photo stability compared to unmodified PerCP	• Allows eBioscience PerCP-Cy5.5 tandems to be used with high power (>15mW) 488nm lasers
• Cy5.5 modification provides increased signal to noise	• Enables detection of less abundant antigens compared to PerCP labeled antibodies
• Smaller, more affordable sizes (25 test for human, and 25 μg for mouse reagents)	• Provides greater budget flexibility

eBioscience PerCP-Cy5.5 conjugates perform!

A comparison study of the leading provider with our newly released material is shown. In this study, hamster anti-mouse CD3ε antibody, (clone 145-2C11) was conjugated with PerCP-Cy5.5 (blue line) and compared to material from the leading provider (solid grey). The results demonstrate that the performance of the two conjugates is essentially indistinguishable.

PerCP-Cy5.5 Specificities

We have an active program to develop reagents to support this fluorophore, so if you can’t find what you’re looking for, please send us a request.

Table 2: Human PerCP-Cy5.5 Specificities
Human PerCP-Cy5.5 Specificities
Specificity	Isotype	Clone	Catalog No.	Size	Price	Size	Price
CD3	Mouse IgG2a	OKT3	45-0037	25 tests	$150	100 tests	$325
CD4	Mouse IgG1	RPA-T4	45-0049	25 tests	$150	100 tests	$325
CD8	Mouse IgG1	RPA-T8	45-0088	25 tests	$150	-	-
CD14	Mouse IgG1	61D3	45-0149	25 tests	$150	-	-
CD19	Mouse IgG1	HIB19	45-0199	25 tests	$150	100 tests	$325
CD20	Mouse IgG2b	2H7	45-0209	25 tests	$150	100 tests	$325
CD25 (IL-2R)	Mouse IgG1	BC96	45-0259	25 tests	$175	100 tests	$420
CD33	Mouse IgG1	WM-53	45-0338	25 tests	$150	-	-
CD38 (costimulatory)	Mouse IgG1	CD28.2	45-0289	25 tests	$150	-	-
CD44	Rat IgG2b	IM7	45-0441	25 ug	$125	100 ug	$300
CD45	Mouse IgG1	HI30	45-0459	25 tests	$150	100 tests	$325
CD45R (B220)	Rat IgG2a	RA3-6B2	45-0452	25 ug	$100	-	-
CD45RA	Mouse IgG2b	HI100	45-0458	25 tests	$150	100 tests	$348
CD90	Mouse IgG1	eBio5E10	45-0909	25 tests	$150	100 tests	$348
CD123 (IL-3Rα)	Mouse IgG1	6H6	45-1239	25 tests	$150	100 tests	$348
CD127	Mouse IgG1	eBioRDR5	45-1278	25 tests	$175	100 tests	$420
Foxp3	Rat IgG2a	PCH101	45-4776	25 tests	$215	100 tests	$395
HLA-DR	Mouse IgG2b	LN3	45-9956	25 tests	$150	100 tests	$348
IFN-γ	Mouse IgG1	4S.B3	45-7319	25 tests	$175	-	-
IL-17A	Mouse IgG1	eBio64DEC17	45-7179	25 tests	$150	100 tests	$348
T-bet	Mouse IgG1	eBio4B10	45-5825	25 ug	$195	100 ug	$395
TLR4	Mouse IgG2a	HTA125	45-9917	25 tests	$150	-	-
TNF-α	Mouse IgG1	MAb11	45-7349	25 tests	$125	100 tests	$300
ZAP-70	Mouse IgG1	1E7.2	45-6695	25 ug	$125	100 ug	$300

Isotype Control	Mouse IgG1	-	45-4714	25 ug	$90	100 ug	$225
Isotype Control	Mouse IgG2a	-	45-4724	25 ug	$90	100 ug	$225
Isotype Control	Mouse IgG2b	-	45-4732	25 ug	$90	100 ug	$225
Isotype Control	Rat IgG2a	-	45-4321	25 ug	$75	-	-
Isotype Control	Rat IgG2b	-	45-4031	25 ug	$75	-	-
Streptavidin	-	-	45-4317	25 ug	$145	-	-

Table 3: Mouse PerCP-Cy5.5 Specificities
Mouse PerCP-Cy5.5 Specificities
Specificity	Isotype	Clone	Catalog No.	Size	Price	Size	Price
CCR7	Rat IgG2a	4B12	45-1971	25 ug	$125	100 ug	$300
CD3e	Armenian Hamster IgG	145-2C11	45-0031	25 ug	$100	-	-
CD4	Rat IgG2a	RM4-5	45-0042	25 ug	$100	-	-
CD5	Rat IgG2a	53-7.3	45-0051	25 ug	$125	100 ug	$300
CD8	Rat IgG2a	53-6.7	45-0081	25 ug	$100	-	-
CD11b	Rat IgG2b	M1/70	45-0112	25 ug	$100	100 ug	$300
CD11c	Armenian Hamster IgG	N418	45-0114	25 ug	$125	100 ug	$300
CD14	Rat IgG2a	Sa2-8	45-0141	25 ug	$125	100 ug	$300
CD19	Rat IgG2a	eBio1D3	45-0193	25 ug	$100	-	-
CD25 (IL-2Rα)	Rat IgG1	PC61.5	45-0251	25 ug	$115	100 ug	$300
CD28	Golden Syrian Hamster IgG	37.51	45-0281	25 ug	$115	100 ug	$300
CD34	Rat IgG2a	RAM34	45-0341	25 ug	$125	100 ug	$300
CD44	Rat IgG2b	IM7	45-0441	25 ug	$125	100 ug	$300
CD45	Rat IgG2b	30-F11	45-0451	25 ug	$100	-	-
CD45R (B220)	Rat IgG2a	RA3-6B2	45-0452	25 ug	$100	-	-
CD45.1	Mouse IgG2a	A20	45-0453	25 ug	$100	-	-
CD45.2	Mouse IgG2a	104	45-0454	25 ug	$100	100 ug	$300
CD90.1	Mouse IgG2a	HIS51	45-0900	25 ug	$100	-	-
CD127 (IL-7Rα)	Rat IgG2a	A7R34	45-1271	25 ug	$125	-	-
F4/80 Antigen	Rat IgG2a	BM8	45-4801	25 ug	$195	100 ug	$365
Foxp3	Rat IgG2a	FJK-16s	45-5773	25 ug	$295	100 ug	$395
IFN-γ	Rat IgG1	XMG1.2	45-7311	25 ug	$100	-	-
IL-2	Rat IgG2b	JES6-5H4	45-7021	25 ug	$125	100 ug	$300
IL-17A	Rat IgG2a	eBio17B7	45-7177	25 ug	$125	100 ug	$300
Ly-6A/E (Sca-1)	Rat IgG2a	D7	45-5981	25 ug	$100	100 ug	$240
Ly-6G (Gr1)	Rat IgG2b	RB6-8C5	45-5931	25 ug	$100	100 ug	$240
NK1.1	Mouse IgG2a	PK136	45-5941	25 ug	$115	100 ug	$300
T-bet	Mouse IgG1	eBio4B10	45-5825	25 ug	$195	100 ug	$395
TCRb	Armenian Hamster IgG	H57-597	45-5961	25 ug	$125	100 ug	$300
TER-119	Rat IgG2b	TER-119	45-5921	25 ug	$125	100 ug	$300
ZAP-70	Mouse IgG1	1E7.2	45-6695	25 ug	$125	100 ug	$300

Isotype Control	Armenian Hamster IgG	eBio299Arm	45-4888	25 ug	$75	-	-
Isotype Control	Mouse IgG1	-	45-4714	25 ug	$90	100 ug	$225
Isotype Control	Mouse IgG2a	-	45-4724	25 ug	$90	100 ug	$225
Isotype Control	Rat IgG1	-	45-4301	25 ug	$75	-	-
Isotype Control	Rat IgG2a	-	45-4321	25 ug	$75	-	-
Isotype Control	Rat IgG2b	-	45-4031	25 ug	$75	-	-

PerCP contained in this product is protected by patents owned by Becton, Dickinson & Company (European patent 0314406, or Japanese Patent JP1888759). This product will not be sold or shipped to customers in France, Germany, Italy, United Kingdom or Japan until the pertinent patents are no longer in effect (October 21, 2008).

Supporting Information

Filter recommendations for PerCP-Cy5.5

About PerCP

Peridinin is a light-harvesting carotenoid, a pigment related to chlorophyll which appears in the Peridinin-chlorophyll-protein (PerCP) light-harvesting complex in dinoflagellates. The PerCP complex is unique in its high ratio of peridinin to chlorophyll; most light-harvesting complexes contain more chlorophyll than other carotenoids, but the PerCP contains eight peridinin and two chlorophyll molecules arranged to promote peridinin-chlorophyll energy transfer. The PerCP complex is a protein trimer with an unusual alpha solenoid protein fold. The spectral properties of PerCP include an exceptionally large Stokes shift (approx. 190nm) making it useful as a third color off 488nm laser-excited measurement systems.

• THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF AMERSHAM PHARMACIA BIOTECH INC. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC. THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH. IT IS NOT LICENSED FOR ANY OTHER USE. THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE. COMMERCIAL USE shall include:

          1. Sale, lease, license or other transfer of the material or any material derived or produced from it.
          2. Sale, lease, license or other grant of rights to use this material or any other material derived or produced from it.
          3. Use of this material to perform services for a fee for third parties.

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• PerCP ribbon graphic sourced from Wikipedia.