Improved PE-Cy7 Conjugations: Brighter Staining, Less Compensation, Greater Stability

The histogram demonstrates staining with new PE-Cy7 anti-human CD14 from eBioscience (red line), or from the competitor (green line). Data provided courtesy of Mihoko Whalen, Tobias Kollmann and Pascal Lavoie from the Child & Family Research Institute in Vancouver.

In the figures below, for each clone indicated, demonstrated on the left is the improved ability to compensate new eBioscience PE-Cy7 conjugates, and on the right is the improved brightness of new eBioscience PE-Cy7 conjugates.

PE-Cy7 anti-mouse CD4, clone GK1.5

BALB/c mouse splenocytes were stained with NEW (left) or OLD (right) PE-Cy7 anti-mouse CD4 (cat. 25-0041). The FL2-%FL3 compensation value was set at 1.4% for both samples.			BALB/c mouse splenocytes were stained with NEW (colored histogram) or OLD (open histogram) PE-Cy7 anti-mouse CD4 (cat. 25-0041). The FL2-%FL3 compensation value was set individually for each sample so that appropriate compensation was achieved.

PE-Cy7 anti-mouse CD62, clone MEL-14

C57Bl/6 mouse splenocytes were stained with NEW (left) or OLD (right) PE-Cy7 anti-mouse CD62 (cat. 25-0621). The FL2-%FL3 compensation value was set at 2.7% for both samples.			C57Bl/6 mouse splenocytes were stained with NEW (colored histogram) or OLD (open histogram) PE-Cy7 anti-mouse CD62 (cat. 25-0621). The FL2-%FL3 compensation value was set individually for each sample so that appropriate compensation was achieved.

PE-Cy7 anti-mouse CD8α, clone 53-6.7

BALB/c mouse splenocytes were stained with NEW (left) or OLD (right) PE-Cy7 anti-mouse CD8α (cat. 25-0081). The FL2-%FL3 compensation value was set at 4.2% for both samples.			BALB/c mouse splenocytes were stained with NEW (colored histogram) or OLD (open histogram) PE-Cy7 anti-mouse CD8α (cat. 25-0081). The FL2-%FL3 compensation value was set individually for each sample so that appropriate compensation was achieved.