Human ILT7 (clone eBio17G10.2): Staining of normal human peripheral blood cells with PE anti-human CD123 (6H6) (cat. 12-1239) and Alexa Fluor® 647 Mouse IgG1, κ Iso Cntrl (cat. 51-4714) (left) or Alexa Fluor® 647 anti-human ILT7, novel pDC marker (right). Cells in the large scatter population were used for analysis.
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Human Foxp3 (clone PCH101): Human PBMCs were surface-stained with FITC anti-CD4 (clone RPA-T4) (cat. 11-0049) and PE anti-CD25 (clone BC96) (cat. 12-0259) and subsequently with 20 μl/million cells Alexa Fluor® 700 anti-human Foxp3 (clone PCH101) or Alexa Fluor® 700 Rat IgG2a Iso Cntrl using the Foxp3 Staining Buffers (cat. 00-5523). The density plot on the left demonstrates co-staining of CD4 and PCH101 while the right plot demonstrates co-staining of CD25 and PCH101. Quadrant lines demarcate the isotype control. Cells in the lymphocyte gate were used for analysis.
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Human IL-15Rα (clone eBioJM7A4): Staining LPS + IFNγ stimulated PBMCs (overnight) with FITC anti-human CD11b (CBRM1/5) (cat. 11-0113) and 0.125 μg of PE Mouse IgG2b Iso Cntrl (cat. 12-4732) (left) or PE anti-human IL-15Rα (right). Cells in the monocyte gate were used for analysis.
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