eBioscience Featured Cytokines:
Cytokines: Ready-SET-Go! ELISA Sets & Kits
New ELISA Kit and Set for Measurement of Human IL-23
The eBioscience Human IL-23 Ready-SET-Go! ELISA Kit and Set contain the necessary reagents, standards, buffers and diluents for performing quantitative enzyme-linked immunosorbent assays (ELISA). These ELISA systems are specifically engineered for accurate and precise measurement of human IL-23 protein levels from samples including serum, plasma, and supernatants from cell cultures.
Unambiguous Quantitation of Human IL-23, Without Interference by IL-12 p70 or p40
The use of a p19-specific capture antibody and a p40-specific detection antibody renders this sandwich ELISA exquisitely specific for human IL-23. IL-12 p40 monomer and IL-12 p70 were run in the assay at 200 ng/ml with no interference or cross-reactivity observed. A panel of 20 unrelated cytokines was run in the IL-23 ELISA at 100 ng/ml with no cross reactivity observed.
Validated Specific Reactivity with Native IL-23
The assays demonstrate parallelism in measuring recombinant and native human IL-23 proteins with a standard curve range of 15 pg/ml to 2,000 pg/ml, and assay sensitivity below 15 pg/ml. The assays have been validated by specific detection of significant levels of native human IL-23 protein in supernatants from a variety of different activated dendritic cell populations.
Human IL-23 and Th17 Cells
IL-23 is a heterodimeric cytokine composed of the p40 subunit of IL-12 disulfide-linked with a protein p19. IL-23 interacts with IL-12Rβ1 and an additional, novel β2-like receptor subunit with STAT4 binding domain, termed IL-23R. IL-23 is secreted by activated dendritic cells. Human IL-23 induces proliferation of memory T cells and induces moderate levels of IFN-γ production by naïve and memory T cells, as compared to IL-12. IL-23-dependent, IL-17-producing CD4+ T cells (Th17 cells) have been identified as a unique subset of Th cells that develops along a pathway that is distinct from the Th1- and Th2- cell differentiation pathways. More recently, de novo differentiation of Th17 cells in the absence of IL-23 has been demonstrated by treatment of naïve CD4 cells with TGF-β1 and IL-6.
New Cytokine ELISA Sets for IL-15, IL-21, IL-23 and IL-29!
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Mouse IL-15 ELISA: Standard curve of mouse IL-15 ELISA Ready-SET-Go! Recombinant cytokine standard concentration is given in pg/ml.
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Human IL-21 ELISA: Standard curve of human IL-21 ELISA Ready-SET-Go! Recombinant cytokine standard concentration is given in pg/ml.
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Human IL-23 ELISA: Standard curve of human IL-23 ELISA Ready-SET-Go! Recombinant cytokine standard concentration is given in pg/ml.
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Human IL-29 ELISA: Standard curve of human IL-29 ELISA Ready-SET-Go! Recombinant cytokine standard concentration is given in pg/ml.
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Capture Antibody: Pre-titrated, purified antibody
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Detection Antibody: Pre-titrated, biotin-conjugated antibody
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Standard: Recombinant cytokine for generating standard curve and calibrating samples
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ELISA/ELISPOT Coating Buffer Powder
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Assay Diluent: 5X concentrated
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Detection enzyme: pre-titrated Avidin-HRP
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Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution
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Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards
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96 Well Plate: Corning Costar 9018 or NUNC Maxisorp flat-bottom (included with catalog numbers ending in suffixes -22, -44, -76, -86)
Contents of Ready-SET-Go! ELISA Kits w/ Pre-Coated Plates
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Pre-coated ELISA Plate
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Detection Antibody: Pre-titrated, biotin-conjugated antibody
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Standard: Recombinant cytokine for generating standard curve and calibrating samples
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Assay Diluent: 5X concentrated
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ELISA Wash Buffer Powder
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Detection enzyme: pre-titrated Avidin-HRP
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Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution
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Stop Solution: 10 mls of 1X solution per plate
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Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards
