Staining of mouse splenocyte cells with 0.125 μg of Biotin Mouse IgG2b Iso Cntrl (cat. 13-4732) (blue histogram) or 0.125 μg of Biotin anti-mouse CD4 (GK1.5)(cat. 13-0041)(purple histogram) followed by eFluor® 650NC Streptavidin. Total viable cells were used for analysis.
Contents: eFluor® 650NC Streptavidin Catalog Number: 95-4317 Concentration: 5 µl Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary Storage Conditions: Store at 2-8°C. DO NOT FREEZE. LIGHT-SENSITIVE MATERIAL.
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95-4317-41
25 tests
95-4317-42
100 tests
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Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.
Description
The streptavidin fluorochrome conjugates are commonly used in indirect staining protocols to detect biotinylated primary antibodies in flow cytometry. Streptavidin binds to biotin with high affinity.
Applications Reported
For research use only, not for diagnostic or therapeutic use. eFluor® 650NC Streptavidin has been reported for use in flow cytometric analysis.
Applications Tested
eFluor® 650NC Streptavidin has been pre-titrated and tested by flow cytometric analysis of mouse or human cells using the eFluor® NC Flow Cytometry Staining Buffer (cat. 00-3222). This can be used at 5 μl per million cells in a 100 μl total staining volume.
Laser/Filter Recommendation: When using eFluor 650NC, we recommend excitation with the 405nm violet laser with an appropriate filter set, such as the 630 LP dichroic mirror with the 660/40 bandpass filter. The eFluor 650NC can be minimally excited off of the 633 nm laser, and because its peak emission is 650nm, it will require some compensation out of the APC detector. Please contact eBioscience Technical Support for more information.
Buffer Recommendation: Comparison of eFluor®NC conjugated antibody staining in different buffers has demonstrated that optimal performance is seen with the eFluor® NC Flow Cytometry Staining Buffer (cat. 00-3222). For a comparison of staining with different buffers, click here.
Click here for answers to questions about fixation and other FAQs in the eFluor® Nanocrystal Frequently Asked Questions.
