Contents: eFluor® 650NC Rat IgG2b Isotype Control Catalog Number: 95-4031 Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary Storage Conditions: Store at 2-8°C. DO NOT FREEZE. LIGHT-SENSITIVE MATERIAL. Host/Isotype: Rat IgG2b, κ
Product Options
Cat. No.
Size
Price
Add Qty to Cart
95-4031-32
125 ul
95-4031-94
500 ul
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
eFluor products are not offered for sale or use in certain regions. Please contact an eBioscience representative for more information on whether our products are available for purchase in your area.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.
Description
The rat IgG2b, κ monoclonal antibody is useful as an isotype control immunoglobulin.
Applications Reported
For research use only, not for diagnostic or therapeutic use. eFluor® 650NC Rat IgG2b Isotype Control has been reported for use in flow cytometric analysis.
Applications Tested
eFluor® 650NC Rat IgG2b Isotype Control has been tested by flow cytometric analysis of mouse splenocytes.
The appropriate volume of isotype control to use is specified on the TDS of the relevant experimental antibody.
Laser/Filter Recommendation: When using eFluor 650NC, we recommend excitation with the 405nm violet laser with an appropriate filter set, such as the 630 LP dichroic mirror with the 660/40 bandpass filter. The eFluor 650NC can be minimally excited off of the 633 nm laser, and because its peak emission is 650nm, it will require some compensation out of the APC detector. Please contact eBioscience Technical Support for more information.
Buffer Recommendation: Comparison of eFluor® NC conjugated antibody staining in different buffers has demonstrated that optimal performance is seen with the eFluor® NC Flow Cytometry Staining Buffer (cat. 00-3222). For a comparison of staining with different buffers, click here.
Click here for answers to questions about fixation and other FAQs in the eFluor® Nanocrystal Frequently Asked Questions.
