Contents: Rat IgG2b K Isotype Control eFluor® 650NC Catalog Number: 95-4031 Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary Storage Conditions: Store at 2-8°C. Light sensitive material. This product is guaranteed for 6 months upon receipt when stored properly. Host/Isotype: Rat IgG2b, κ
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95-4031-32
125 ul
95-4031-94
500 ul
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
eFluor® products are not offered for sale or use in certain regions. Please contact an eBioscience representative for product availability.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The rat IgG2b, κ monoclonal antibody is useful as an isotype control immunoglobulin.
Applications Reported
For research use only, not for diagnostic or therapeutic use. eFluor® 650NC Rat IgG2b Isotype Control has been reported for use in flow cytometric analysis.
Applications Tested
eFluor® 650NC Rat IgG2b Isotype Control has been tested by flow cytometric analysis of mouse splenocytes.
The appropriate volume of isotype control to use is specified on the TDS of the relevant experimental antibody.
Laser/Filter Recommendation: When using eFluor 650NC, we recommend excitation with the 405nm violet laser with an appropriate filter set, such as the 630 LP dichroic mirror with the 660/40 bandpass filter. The eFluor 650NC can be minimally excited off of the 633 nm laser, and because its peak emission is 650nm, it will require some compensation out of the APC detector. Please contact eBioscience Technical Support for more information.
Buffer Recommendation: Comparison of eFluor® NC conjugated antibody staining in different buffers has demonstrated that optimal performance is seen with the eFluor® NC Flow Cytometry Staining Buffer (cat. 00-3222). For a comparison of staining with different buffers, refer to eFluor® Nanocrystals page.
