Product Information

Contents: eFluor^TM 625^NC Mouse IgM Isotype Control
Catalog Number: 94-4752
Sizes: 125 ul, 500 ul
Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Isotype: Mouse IgM, λ

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-4752	Affinity Purified Mouse IgM Isotype Control	N/A	N/A	FC
16-4752	Functional Grade* Purified Mouse IgM Isotype Control	N/A	N/A	FC
11-4752	FITC Mouse IgM Isotype Control	488	518	FC
12-4752	PE Mouse IgM Isotype Control	488	575	FC
57-4752	Pacific Blue® Mouse IgM Isotype Control	402	421	FC
94-4752	eFluor^TM 625^NC Mouse IgM Isotype Control	350-500	625	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The mouse IgM, λ is useful as an isotype control immunoglobulin. The immunoglobulin was generated by immunization with lipopolysaccharide (LPS). The immunoglobulin is reactive with LPS.

Applications Reported

For research use only, not for diagnostic or therapeutic use. eFluor™ 625NC Mouse IgM Isotype Control has been reported for use in flow cytometric analysis.

Applications Tested

eFluor^TM 625^NC Mouse IgM Isotype Control has been tested by flow cytometric analysis of normal human peripheral blood cells.

The appropriate volume of isotype control to use is specified on the TDS of the relevant experimental antibody.

Laser/Filter Recommendation: When using eFluor^TM 625^NC, we recommend excitation with the 405nm violet laser with an appropriate filter set, such as the 595LP dichroic mirror with the 605/50 bandpass filter. An acceptable alternative is the 625/20 bandpass filter. Please contact eBioscience Technical Support for more information.

Buffer Recommendation: Comparison of eFluor^TM Nanocrystal conjugated antibody staining in different buffers has demonstrated that optimal performance is seen with the eFluor^TM NC Flow Cytometry Staining Buffer (cat. 00-3222). For a comparison of staining with different buffers, click here.

Whole Blood Recommendation: If staining whole blood samples, collection into heparin or sodium citrate-containing tubes is recommended. If EDTA is being used as an anti-coagulant, lysis of RBCs with eBioscience 1X RBC Lysis Buffer (cat. 00-4333) before staining is required.

Click here to view our list of eFluor^TM Nanocrystal Frequently Asked Questions.

Related Products

Cat. 00-3222 eFluor™ NC Flow Cytometry Staining Buffer