Product Information

Contents: eFluor^TM 625^NC anti-human CD20
Catalog Number: 94-0209
Sizes: 25 tests, 100 tests
Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 2H7
Isotype: Mouse IgG2b, κ
HLDA No.: IV B201

Staining of normal human peripheral blood cells with Pacific Blue anti-human CD3 (OKT3) (cat. 57-0037) and eFluor™ 625^NC Mouse IgG2b Isotype Control (cat. 94-4732) (left) or eFluor™ 625^NC anti-human CD20 (2H7) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0209	Affinity Purified anti-human CD20	N/A	N/A	FC IH/F
13-0209	Biotin anti-human CD20	N/A	N/A	FC IH/F
11-0209	FITC anti-human CD20	488	518	FC
12-0209	PE anti-human CD20	488	575	FC
15-0209	PE-Cy5 anti-human CD20	488	670	FC
17-0209	Allophycocyanin (APC) anti-human CD20	633	660	FC
25-0209	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD20	488	760	FC
56-0209	Alexa Fluor® 700 anti-human CD20 (APC-Cy5.5 Equivalent)	633	723	FC
45-0209	PerCP-Cy5.5 anti-human CD20 (PE-Cy5.5 replacement)	488	690	FC
94-0209	eFluor^TM 625^NC anti-human CD20	350-500	625	FC
95-0209	eFluor^TM 650^NC anti-human CD20	350-500	650	FC
47-0209	Allophycocyanin-eFluor^TM 780 anti-human CD20 (APC-Alexa Fluor® 750 replacement)	633	780	FC
48-0209	eFluor^TM 450 anti-human CD20	405	450	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 2H7 monoclonal antibody reacts with human CD20, a 33-36 kDa transmembrane protein. CD20 is expressed by developing B cells as well as mature B cells but not plasma cells. CD20 has been detected at low levels on a small subset of mature T cells. It is suggested that CD20 plays a role in B-cell activation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 2H7 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This 2H7 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells using eFluor^TM NC Flow Cytometry Staining Buffer (cat. 00-3222). This can be used at 5μL/test (one million cells in a 100μL total staining volume).

The isotype control eFluor^TM 625^NC mouse IgG2b (cat. 94-4732) should be used at 5μL/test.

Laser/Filter Recommendation: When using eFluor^TM 625^NC, we recommend excitation with the 405nm violet laser with an appropriate filter set, such as the 595LP dichroic mirror with the 605/50 bandpass filter. An acceptable alternative is the 625/20 bandpass filter. Please contact eBioscience Technical Support for more information.

Buffer Recommendation: Comparison of eFluor^TM Nanocrystal conjugated antibody staining in different buffers has demonstrated that optimal performance is seen with the eFluor^TM NC Flow Cytometry Staining Buffer (cat. 00-3222). For a comparison of staining with different buffers, click here.

Whole Blood Recommendation: If staining whole blood samples, collection into heparin or sodium citrate-containing tubes is recommended. If EDTA is being used as an anti-coagulant, lysis of RBCs with eBioscience 1X RBC Lysis Buffer (cat. 00-4333) before staining is required.

Click here to view our list of eFluor^TM Nanocrystal Frequently Asked Questions.

References

Reinherz, E.L., et al. eds. 1985. Leukocyte Typing II (Vol. I, II, and III). Human Leukocyte Differentiation Antigens detected by Monoclonal Antibodies.
McMichael, A.J., P.C.L. Beverly, et al. eds. 1987. Leucocyte Typing III: White Cell Differentiation Antigens. Oxford University Press. New York.
Knapp, W., B. Dorken, et al. eds. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
Schlossman, S., L. Bloumsell, et al. eds. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.

Related Products

Cat. 00-3222 eFluor™ NC Flow Cytometry Staining Buffer
Cat. 94-4732 eFluor™ 625NC Mouse IgG2b Isotype Control