Product Information

Contents: eFluor^TM 625^NC anti-human CD15
Catalog Number: 94-0159
Sizes: 25 tests, 100 tests
Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: HI98
Isotype: Mouse IgM, κ
HLDA No.: IV M141

Staining of normal human peripheral blood cells with eFluor™ 625^NC Mouse IgM Isotype Control (cat. 94-4752) (blue) or eFluor™ 625^NC anti-human CD15 (HI98) (purple). Cells in the granulocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0159	Affinity Purified anti-human CD15	N/A	N/A	FC IH/F
13-0159	Biotin anti-human CD15	N/A	N/A	FC IH/F
11-0159	FITC anti-human CD15	488	518	FC
57-0159	Pacific Blue® anti-human CD15	402	421	FC
94-0159	eFluor^TM 625^NC anti-human CD15	350-500	625	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The HI98 monoclonal antibody reacts with human CD15, Lewis X. This 3-fucosyl-N-acetyllactosamine carbohydrate moiety is expressed by granulocytes. Monocytes express this structure at varying degree while lymphocytes are negative.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This HI98 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This HI98 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood cells using the eFluor^TM NC Flow Cytometetry Staining Buffer (cat. 00-3222). This can be used at 5 μl/test (one million cells in a 100μL total staining volume).

The isotype control eFluor^TM 625^NC mouse IgM (cat 94-4752) should be used at 5μL/test.

Laser/Filter Recommendation: When using eFluor^TM 625^NC, we recommend excitation with the 405nm violet laser with an appropriate filter set, such as the 595LP dichroic mirror with the 605/50 bandpass filter. An acceptable alternative is the 625/20 bandpass filter. Please contact eBioscience Technical Support for more information.

Buffer Recommendation: Comparison of eFluor^TM Nanocrystal conjugated antibody staining in different buffers has demonstrated that optimal performance is seen with the eFluor^TM NC Flow Cytometry Staining Buffer (cat. 00-3222). For a comparison of staining with different buffers, click here.

Whole Blood Recommendation: If staining whole blood samples, collection into heparin or sodium citrate-containing tubes is recommended. If EDTA is being used as an anti-coagulant, lysis of RBCs with eBioscience 1X RBC Lysis Buffer (cat. 00-4333) before staining is required.

Click here to view our list of eFluor^TM Nanocrystal Frequently Asked Questions.

References

Knapp, W., B. Dorken, et al. eds. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.

Related Products

Cat. 00-3222 eFluor™ NC Flow Cytometry Staining Buffer
Cat. 94-4752 eFluor™ 625NC Mouse IgM Isotype Control