Product Information

Contents: eFluor^TM 605^NC anti-human HLA-DR
Catalog Number: 93-9956
Sizes: 25 tests, 100 tests
Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: LN3
Isotype: Mouse IgG2b, κ

Staining of normal human peripheral blood cells with eFluor^TM 605^NC Mouse IgG2b Iso Cntrl (cat. 53-4732) (blue) or eFluor^TM 605^NC anti-human HLA-DR (LN3) (purple). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9956	Affinity Purified anti-human HLA-DR	N/A	N/A	FC IHC
13-9956	Biotin anti-human HLA-DR	N/A	N/A	FC
11-9956	Fluorescein isothiocyanate (FITC) anti-human HLA-DR	488	518	FC
12-9956	PE anti-human HLA-DR	488	575	FC
15-9956	Phycoerythrin-Cy5 (PE-Cy5) anti-human HLA-DR	488	670	FC
17-9956	APC anti-human HLA-DR	633	660	FC
25-9956	Phycoerythrin-Cy7 (PE-Cy7) anti-human HLA-DR	488	760	FC
56-9956	Alexa Fluor® 700 anti-human HLA-DR (APC-Cy5.5 Equivalent)	633	723	FC
27-9956	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-human HLA-DR	633	779	FC
45-9956	PerCP-Cy5.5 anti-human HLA-DR (PE-Cy5.5 replacement)	488	690	FC
93-9956	eFluor^TM 605^NC anti-human HLA-DR	350-500	605	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The LN3 mAb reacts with the human major histocompatibility complex (MHC) class II, HLA-DR. HLA-DR is expressed on the surface of human antigen presenting cells (APC) including B cells, monocytes, macrophages, DCs, and activated T cells. HLA-DR is a heterodimeric transmembrane protein composed of α and β subunits and plays an important role in the presentation of peptides to CD4⁺ T lymphocytes.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This LN3 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This LN3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells using eFluor^TM NC Flow Cytometry Staining Buffer (cat. 00-3222). This can be used at 5 μl/test (one million cells in a 100μL total staining volume).

The isotype control eFluor^TM 605^NC mouse IgG2b (cat. 93-4732) should be used at 5μL/test.

Laser/Filter Recommendation: When using eFluor^TM 605^NC, we recommend excitation with the 405nm violet laser with an appropriate filter set, such as the 595LP dichroic mirror with the 605/40 bandpass filter. An acceptable alternative is the 610/20 bandpass filter. Please contact eBioscience Technical Support for more information.

Buffer Recommendation: Comparison of eFluor^TM Nanocrystal conjugated antibody staining in different buffers has demonstrated that optimal performance is seen with the eFluor^TM NC Flow Cytometry Staining Buffer (cat. 00-3222). For a comparison of staining with different buffers, click here.

Whole Blood Recommendation: If staining whole blood samples, collection into heparin or sodium citrate-containing tubes is recommended. If EDTA is being used as an anti-coagulant, lysis of RBCs with eBioscience 1X RBC Lysis Buffer (cat. 00-4333) before staining is required.

Click here to view our list of eFluor^TM Nanocrystal Frequently Asked Questions.

References

Fullen, D. R. and J. T. Headington (1998). "Factor XIIIa-positive dermal dendritic cells and HLA-DR expression in radial versus vertical growth-phase melanomas [see comments]." J Cutan Pathol 25(10): 553-8.
Hua, Z. X., K. E. Tanaka, et al. (1998). "Immunoreactivity for LN2 and LN3 distinguishes small cell carcinomas from non-small cell carcinomas in the lung." Hum Pathol 29(12): 1441-6.
Ioachim, H. L., S. E. Pambuccian, et al. (1996). "Lymphoid monoclonal antibodies reactive with lung tumors. Diagnostic applications." Am J Surg Pathol 20(1): 64-71.
Davey, F. R., S. Olson, et al. (1988). "The immunophenotyping of extramedullary myeloid cell tumors in paraffin-embedded tissue sections." Am J Surg Pathol 12(9): 699-707.
Norton, A. J. and P. G. Isaacson (1987). "Detailed phenotypic analysis of B-cell lymphoma using a panel of antibodies reactive in routinely fixed wax-embedded tissue." Am J Pathol 128(2): 225-40.

Related Products

Cat. 00-3222 eFluor™ NC Flow Cytometry Staining Buffer