Product Information
Contents: eFluorTM 605NC Mouse IgG1, Isotype Control
Catalog Number: 93-4714
Sizes: 125 ul, 500 ul
Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Isotype: Mouse IgG1, κ
|
|
|
| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 14-4714 |
Affinity Purified Mouse IgG1, K Isotype Control |
N/A |
N/A |
FC
IC Flow
IP
WB
|
| 16-4714 |
Functional Grade* Purified Mouse IgG1, K Isotype Control |
N/A |
N/A |
FC
|
| 13-4714 |
Biotin Mouse IgG1, K Isotype Control |
N/A |
N/A |
FC
|
| 11-4714 |
Fluorescein isothiocyanate (FITC) Mouse IgG1, K Isotype Control |
488 |
518 |
FC
|
| 12-4714 |
Phycoerythrin (PE) Mouse IgG1, K Isotype Control |
488 |
575 |
FC
|
| 15-4714 |
Phycoerythrin-Cy5 (PE-Cy5) Mouse IgG1, K Isotype Control |
488 |
670 |
FC
|
| 17-4714 |
Allophycocyanin (APC) Mouse IgG1, K Isotype Control |
633 |
660 |
FC
|
| 25-4714 |
Phycoerythrin-Cy7 (PE-Cy7) Mouse IgG1, K Isotype Control |
488 |
760 |
FC
|
| 35-4714 |
Phycoerythrin-Cy5.5 (PE-Cy5.5) Mouse IgG1, K Isotype Control |
488 |
690 |
FC
|
| 51-4714 |
Alexa Fluor® 647 Mouse IgG1, K Isotype Control |
633 |
668 |
FC
|
| 53-4714 |
Alexa Fluor® 488 Mouse IgG1, K Isotype Control |
488 |
519 |
FC
|
| 56-4714 |
Alexa Fluor® 700 Mouse IgG1, K Isotype Control (APC-Cy5.5 Equivalent) |
633 |
723 |
FC
|
| 57-4714 |
Pacific Blue® Mouse IgG1, K Isotype Control |
402 |
421 |
FC
|
| 27-4714 |
Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) Mouse IgG1, K Isotype Control (APC-Cy7 Equivalent) |
633 |
779 |
FC
|
| 45-4714 |
PerCP-Cy5.5 Mouse IgG1, K Isotype Control |
488 |
690 |
FC
|
| 93-4714 |
eFluorTM 605NC Mouse IgG1, Isotype Control |
350-500 |
605 |
FC
|
| 94-4714 |
eFluorTM 625NC Mouse IgG1 Isotype Control |
350-500 |
625 |
FC
|
| 95-4714 |
eFluorTM 650NC Mouse IgG1, K Isotype Control |
350-500 |
650 |
FC
|
| 47-4714 |
Allophycocyanin-eFluorTM 780 Mouse IgG1, K Isotype Control (APC-Alexa Fluor® 750 replacement) |
633 |
780 |
FC
|
| 48-4714 |
eFluorTM 450 Mouse IgG1, K Isotype Control (Pacific Blue® replacement) |
405 |
450 |
FC
|
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested. |
Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively. |
eFluor products are not offered for sale or use in certain regions. Please contact an eBioscience representative for more information on whether our products are available for purchase in your area.
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The monoclonal mouse IgG1, κ, immunoglobulin is useful as an isotype control.
Applications Reported
For research use only, not for diagnostic or therapeutic use. eFluor™ 605
NC Mouse IgG1, Isotype Control has been reported for use in flow cytometric analysis.
Applications Tested
eFluor™ 605
NC Mouse IgG1, Isotype Control has been tested by flow cytometric analysis of normal human peripheral blood cells.
The appropriate volume of isotype control to use is specified on the TDS of the relevant experimental antibody.
Laser/Filter Recommendation: When using eFluor
TM 605
NC, we recommend excitation with the 405nm violet laser with an appropriate filter set, such as the 595LP dichroic mirror with the 605/40 bandpass filter. An acceptable alternative is the 610/20 bandpass filter. Please contact
eBioscience Technical Support for more information.
Buffer Recommendation: Comparison of eFluor
TM Nanocrystal conjugated antibody staining in different buffers has demonstrated that optimal performance is seen with the eFluor
TM NC Flow Cytometry Staining Buffer (cat.
00-3222). For a comparison of staining with different buffers, click
here.
Whole Blood Recommendation: If staining whole blood samples, collection into heparin or sodium citrate-containing tubes is recommended. If EDTA is being used as an anti-coagulant, lysis of RBCs with eBioscience 1X RBC Lysis Buffer (cat.
00-4333) before staining is required.
Click here to view our list of eFluorTM Nanocrystal Frequently Asked Questions.
Related Products
Cat. 00-3222 eFluor™ NC Flow Cytometry Staining Buffer
