IgG2a

Contents: eFluor^TM 605^NC Rat IgG2a Isotype Control
Catalog Number: 93-4321
Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Isotype: Rat IgG2a, κ

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
93-4321-32	125 ul	$75
93-4321-94	500 ul	$180

*International customers: Please contact your distributor for region specific pricing.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-4321	Affinity Purified Rat IgG2a Isotype Control	N/A	N/A	FC
16-4321	Functional Grade* Purified Rat IgG2a Isotype Control	N/A	N/A	FC
13-4321	Biotin Rat IgG2a Isotype Control	N/A	N/A	FC
11-4321	FITC Rat IgG2a Isotype Control	488	518	FC
12-4321	PE Rat IgG2a Isotype Control	488	575	FC
15-4321	PE-Cy5 Rat IgG2a Isotype Control	488	670	FC
17-4321	APC Rat IgG2a Isotype Control	633	660	FC
10-4321	May be discontinued; see superior product APC-Alexa Fluor® 750 Allophycocyanin-Cy7 (APC-Cy7) Rat IgG2a Isotype Control	633	760	FC
25-4321	Phycoerythrin-Cy7 (PE-Cy7) Rat IgG2a Isotype Control	488	760	FC
30-4321	DISCONTINUED - Allophycocyanin-Cy5.5 (APC-Cy5.5) Rat IgG2a Isotype Control (Alexa Fluor® 700 Equivalent)	633	690	FC
35-4321	PE-Cy5.5 Rat IgG2a Isotype Control	488	690	FC
72-4321	Phycoerythrin (PE) Set Rat IgG2a Isotype Control	488	575	IC Flow
51-4321	Alexa Fluor® 647 Rat IgG2a Isotype Control	633	668	FC
53-4321	Alexa Fluor® 488 Rat IgG2a Isotype Control	488	519	FC
56-4321	Alexa Fluor® 700 Rat IgG2a Isotype Control (APC-Cy5.5 Equivalent)	633	723	FC
57-4321	Pacific Blue® Rat IgG2a Isotype Control	402	421	FC
27-4321	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) Rat IgG2a Isotype Control (APC-Cy7 Equivalent)	633	779	FC
45-4321	PerCP-Cy5.5 Rat IgG2a Isotype Control	488	690	FC
93-4321	eFluor^TM 605^NC Rat IgG2a Isotype Control	350-500	605	FC
94-4321	eFluor^TM 625^NC Rat IgG2a Isotype Control	350-500	625	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

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Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The monoclonal rat IgG2a, κ is useful as an isotype control immunoglobulin.

Applications Reported

For research use only, not for diagnostic or therapeutic use. eFluor™ 605^NC Rat IgG2a Isotype Control has been reported for use in flow cytometric analysis.

Applications Tested

eFluor^TM 605^NC Rat IgG2a Isotype Control has been tested by flow cytometric analysis of mouse splenocytes.

The appropriate volume of isotype control to use is specified on the TDS of the relevant experimental antibody.

Laser/Filter Recommendation: When using eFluor^TM 605^NC, we recommend excitation with the 405nm violet laser with an appropriate filter set, such as the 595LP dichroic mirror with the 605/40 bandpass filter. An acceptable alternative is the 610/20 bandpass filter. Please contact eBioscience Technical Support for more information.

Buffer Recommendation: Comparison of eFluor^TM Nanocrystal conjugated antibody staining in different buffers has demonstrated that optimal performance is seen with the eFluor^TM NC Flow Cytometry Staining Buffer (cat. 00-3222). For a limited time the eFluor^TM NC Flow Cytometry Staining Buffer will be provided complimentary with the purchase of eFluor^TM Nanocrystal conjugated products. For a comparison of staining with different buffers, click here.

Whole Blood Recommendation: If staining whole blood samples, collection into heparin or sodium citrate-containing tubes is recommended. If EDTA is being used as an anti-coagulant, lysis of RBCs with eBioscience 1X RBC Lysis Buffer (cat. 00-4333) before staining is required.

Click here to view our list of eFluor^TM Nanocrystal Frequently Asked Questions.

Related Products

Cat. 00-3222 eFluor™ NC Flow Cytometry Staining Buffer