Product Information

Contents: eFluor^TM 605^NC Streptavidin conjugate
Catalog Number: 93-4317
Sizes: 25 tests, 100 tests
Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.

Staining of mouse splenocyte cells with 0.125 µg of Biotin Mouse IgG2b Iso Cntrl (cat. 13-4732) (blue histogram) or 0.125 µg of Biotin anti-mouse CD4 (GK1.5)(cat. 13-0041)(purple histogram) followed by eFluor 605^NC Streptavidin. Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-4317	Streptavidin-FITC (Fluorescein isothiocyanate)	488	518	FC
12-4317	Streptavidin-PE (Phycoerythrin)	488	575	FC
15-4317	Streptavidin-PE-Cy5 (Phycoerythrin-Cy5)	488	670	FC
17-4317	Streptavidin Allophycocyanin (SA-APC)	633	660	FC
19-4317	Streptavidin-Cy5	633	670	FC
25-4317	Streptavidin-PE-Cy7 (Phycoerythrin-Cy7)	488	760	FC
35-4317	Streptavidin-PE-Cy5.5	488	690	FC
45-4317	Streptavidin-PerCP-Cy5.5 (PE-Cy5.5 replacement)	488	690	FC
93-4317	eFluor^TM 605^NC Streptavidin conjugate	350-500	605	FC
94-4317	eFluor^TM 625^NC Streptavidin conjugate	350-500	625	FC
95-4317	eFluor^TM 650^NC Streptavidin	350-500	650	FC
47-4317	Allophycocyanin-eFluor^TM 780 Streptavidin (APC-Alexa Fluor® 750 replacement)	633	780	FC
48-4317	eFluor^TM 450 Streptavidin (Pacific Blue® replacement)	405	450	FC
49-4317	eFluor^TM 710 Streptavidin (APC-Cy5.5 replacement)	633	710	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The streptavidin fluorochrome conjugates are commonly used in indirect staining protocols to detect biotinylated primary antibodies in flow cytometry. Streptavidin binds to biotin with high affinity.

Applications Reported

For research use only, not for diagnostic or therapeutic use. eFluor™ 605NC Streptavidin has been reported for use in flow cytometric analysis.

Applications Tested

eFluor^TM 605^NC Streptavidin has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells using the eFluor NC Flow Cytometry Staining Buffer (cat. 00-3222).

This can be used at 5μL/test (one million cells in a 100μL total staining volume).

Laser/Filter Recommendation: When using eFluor^TM 605^NC, we recommend excitation with the 405nm violet laser with an appropriate filter set, such as the 595LP dichroic mirror with the 605/40 bandpass filter. An acceptable alternative is the 610/20 bandpass filter. Please contact eBioscience Technical Support for more information.

Buffer Recommendation: Comparison of eFluor^TM Nanocrystal conjugated antibody staining in different buffers has demonstrated that optimal performance is seen with the eFluor^TM NC Flow Cytometry Staining Buffer (cat. 00-3222). For a comparison of staining with different buffers, click here.

Whole Blood Recommendation: If staining whole blood samples, collection into heparin or sodium citrate-containing tubes is recommended. If EDTA is being used as an anti-coagulant, lysis of RBCs with eBioscience 1X RBC Lysis Buffer (cat. 00-4333) before staining is required.

Click here to view our list of eFluor^TM Nanocrystal Frequently Asked Questions.

Related Products

Cat. 13-0048 Biotin anti-human CD4 (clone OKT4 (OKT-4))
Cat. 00-3222 eFluor™ NC Flow Cytometry Staining Buffer