Staining of mouse splenocyte cells with 0.125 µg of Biotin Mouse IgG2b Iso Cntrl (cat. 13-4732) (blue histogram) or 0.125 µg of Biotin anti-mouse CD4 (GK1.5)(cat. 13-0041)(purple histogram) followed by eFluor 605NC Streptavidin. Total viable cells were used for analysis.
Contents: eFluor® 605NC Streptavidin conjugate Catalog Number: 93-4317 Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary Storage Conditions: Store at 4°C. DO NOT FREEZE. LIGHT-SENSITIVE MATERIAL.
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Size
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93-4317-41
25 tests
93-4317-42
100 tests
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Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.
Description
The streptavidin fluorochrome conjugates are commonly used in indirect staining protocols to detect biotinylated primary antibodies in flow cytometry. Streptavidin binds to biotin with high affinity.
Applications Reported
For research use only, not for diagnostic or therapeutic use. eFluor™ 605NC Streptavidin has been reported for use in flow cytometric analysis.
Applications Tested
eFluor 605NC Streptavidin has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells using the eFluor NC Flow Cytometry Staining Buffer (cat. 00-3222).
This can be used at 5μL/test (one million cells in a 100μL total staining volume).
Laser/Filter Recommendation: When using eFluor 605NC, we recommend excitation with the 405nm violet laser with an appropriate filter set, such as the 595LP dichroic mirror with the 605/40 bandpass filter. An acceptable alternative is the 610/20 bandpass filter. For instruments not equipped with a violet laser, the eFluor 605NC is also excited by the 488 nm blue laser and can be used as a PE-Texas Red alternative. Please contact eBioscience Technical Support for more information.
Buffer Recommendation: Comparison of eFluor Nanocrystal conjugated antibody staining in different buffers has demonstrated that optimal performance is seen with the eFluor NC Flow Cytometry Staining Buffer (cat. 00-3222). For a comparison of staining with different buffers, click here.
Whole Blood Recommendation: If staining whole blood samples, collection into heparin or sodium citrate-containing tubes is recommended. If EDTA is being used as an anti-coagulant, lysis of RBCs with eBioscience 1X RBC Lysis Buffer (cat. 00-4333) before staining is required.
Click here to view our list of eFluor Nanocrystal Frequently Asked Questions.
