Product Information

Contents: Streptavidin eFluor^TM 605^NC (IF)
Catalog Number: 93-2317
Sizes: 100 ul
Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.

Frozen sections of mouse lymph node were stained with either no primary antibody (left panel) or Biotin anti-mouse CD3 (145-2C11) (cat. 13-0031) (right panel) and subsequently with a 1:50 dilution of Streptavidin eFluor^TM 605^NC (IF) to visualize the T cell zone. FITC anti-mouse B220 (RA3-6B2) (cat. 11-0452) was used to identify B cell follicles.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
93-2317	eFluor^TM 605^NC streptavidin conjugate (IF)	350-500	605	IHC
95-2317	eFluor^TM 650^NC streptavidin conjugate (IF)	350-500	650	MIC

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Description

The streptavidin fluorochrome conjugates are commonly used in indirect staining protocols to detect biotinylated primary antibodies in flow cytometry and immunofluorescent microscopy. Streptavidin binds to biotin with high affinity.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eFluor™ 605^NC streptavidin conjugate (IF) has been reported for use in immunofluorescent microscopy.

Applications Tested

Streptavidin eFluor^TM 605^NC (IF) has been tested by immunofluorescence microscopy with a biotinylated primary antibody. This eFluor^TM 605^NC has been specially formulated for use in immunofluorescent microscopy. As a starting concentration, this can be used at a 1:50 - 1:100 dilution. However, it is recommended that the antibody be carefully titrated for optimal performance. Please contact eBioscience Technical Support for more information.

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