Product Information

Contents: eFluor^TM 605^NC anti-mouse CD86 (B7-2, B72)
Catalog Number: 93-0862
Sizes: 25 tests, 100 tests
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: GL1
Isotype: Rat IgG2a, κ

Staining of LPS stimulated BALB/c splenocytes with Pacific Blue anti-mouse/human CD11b (M1/70) (cat. 57-0112) and ^TM Rat IgG2a Isotype Control (cat. 93-4321) (left) or ^TM anti-mouse CD86 (GL-1) (right). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0862	Affinity Purified anti-mouse CD86 (B7-2, B72)	N/A	N/A	FA FC IH/F IP
16-0862	Functional Grade* Purified anti-mouse CD86 (B7-2, B72)	N/A	N/A	FA FC
13-0862	Biotin anti-mouse CD86 (B7-2, B72)	N/A	N/A	FC
11-0862	FITC anti-mouse CD86 (B7-2, B72)	488	518	FC
12-0862	PE anti-mouse CD86 (B7-2, B72)	488	575	FC
15-0862	PE-Cy5 anti-mouse CD86 (B7-2, B72)	488	670	FC
17-0862	APC anti-mouse CD86 (B7-2, B72)	633	660	FC
93-0862	eFluor^TM 605^NC anti-mouse CD86 (B7-2, B72)	350-500	605	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The GL1 monoclonal antibody reacts with mouse CD86, an ~80 kDa surface receptor also known as B7-2. CD86 & CD80 are members of the B7 family of costimulatory molecules. CD86 is expressed at low level on B cells, macrophages, and dendritic cells and is upregulated on B cells through a variety of surface stimuli including the BCR complex, CD40 and some cytokine receptors. CD86 is also expressed by activated mouse T cells and thioglycolate-elicited peritoneal cells. In addition to CD80 (B7-1), CD86 is a counter-receptor for the T cell surface molecules CD28 and CD152 (CTLA-4). This interaction plays a critical role in T-B crosstalk, T cell costimulation, autoantibody production and Th2-mediated Ig production. The kinetics of upregulation of CD86 upon stimulation, supports its major contribution during the primary phase of an immune response.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This GL1 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This GL1 antibody has been pre-titrated and tested by flow cytometric analysis of LPS stimulated mouse splenocytes using eFluor^TM NC Flow Cytometry Staining Buffer (cat. 00-3222). This can be used at 5 μl per million cells in a 100 μl total staining volume.

The isotype control eFluor^TM 605^NC rat IgG2a (cat. 93-4321) should be used at 5μl/test.

Laser/Filter Recommendation: When using eFluor^TM 605^NC, we recommend excitation with the 405nm violet laser with an appropriate filter set, such as the 595LP dichroic mirror with the 605/40 bandpass filter. An acceptable alternative is the 610/20 bandpass filter. Please contact eBioscience Technical Support for more information.

Buffer Recommendation: Comparison of eFluor^TM Nanocrystal conjugated antibody staining in different buffers has demonstrated that optimal performance is seen with the eFluor^TM NC Flow Cytometry Staining Buffer (cat. 00-3222). For a comparison of staining with different buffers, click here.

Whole Blood Recommendation: If staining whole blood samples, collection into heparin or sodium citrate-containing tubes is recommended. If EDTA is being used as an anti-coagulant, lysis of RBCs with eBioscience 1X RBC Lysis Buffer (cat. 00-4333) before staining is required.

Click here to view our list of eFluor^TM Nanocrystal Frequently Asked Questions.

References

Hathcock, K. S., G. Laszlo, et al. (1993). "Identification of an alternative CTLA-4 ligand costimulatory for T cell activation [see comments]." Science 262(5135): 905-7.
Freeman, G. J., F. Borriello, et al. (1993). "Murine B7-2, an alternative CTLA4 counter-receptor that costimulates T cell proliferation and interleukin 2 production." J Exp Med 178(6): 2185-92.
Inaba, K., M. Witmer-Pack, et al. (1994). "The tissue distribution of the B7-2 costimulator in mice: abundant expression on dendritic cells in situ and during maturation in vitro." J Exp Med 180(5): 1849-60.
Hathcock, K. S., G. Laszlo, et al. (1994). "Comparative analysis of B7-1 and B7-2 costimulatory ligands: expression and function." J Exp Med 180(2): 631-40

Related Products

Cat. 93-4321 eFluor™ 605NC Rat IgG2a Isotype Control