Product Information

Contents: eFluor^TM 605^NC anti-mouse CD69 (Very Early Activation Antigen, VEA)
Catalog Number: 93-0691
Sizes: 25 tests, 100 tests
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: H1.2F3
Isotype: Armenian Hamster IgG

Staining of ConA stimulated mouse splenocytes with APC anti-mouse CD25 (PC61.5) (cat. 17-0251) and eFluor^TM 605^NC Armenian Hamster IgG Isotype Control (cat. 93-4888) (left) or eFluor^TM 605^NC anti-mouse CD69 (H1.2F3) (right). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0691	Affinity Purified anti-mouse CD69 (Very Early Activation Antigen, VEA)	N/A	N/A	FA FC IHC IP
16-0691	Functional Grade* Purified anti-mouse CD69 (Very Early Activation Antigen, VEA)	N/A	N/A	FA FC
13-0691	Biotin anti-mouse CD69 (Very Early Activation Antigen, VEA)	N/A	N/A	FC
11-0691	FITC anti-mouse CD69 (Very Early Activation Antigen, VEA)	488	518	FC
12-0691	PE anti-mouse CD69 (Very Early Activation Antigen, VEA)	488	575	FC
15-0691	PE-Cy5 anti-mouse CD69 (Very Early Activation Antigen, VEA)	488	670	FC
17-0691	Allophycocyanin (APC) anti-mouse CD69 (Very Early Activation Antigen, VEA)	633	660	FC
25-0691	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse CD69 (Very Early Activation Antigen, VEA)	488	760	FC
45-0691	PerCP-Cy5.5 anti-mouse CD69 (Very Early Activation Antigen, VEA) (PE-Cy5.5 replacement)	488	690	FC
93-0691	eFluor^TM 605^NC anti-mouse CD69 (Very Early Activation Antigen, VEA)	350-500	605	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The H1.2F3 monoclonal antibody reacts with mouse CD69, also known as very early activation antigen (VEA). CD69 is approximately 35 kDa and is expressed on the surface as a disulfide-linked dimer. While a small subset of lymphocytes in the thymus, spleen and lymph nodes express this antigen, activation of both T and B cells rapidly upregulates the surface expression of CD69, suggesting a role for CD69 in lymphocyte development and activation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This H1.2F3 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This H1.2F3 antibody has been pre-titrated and tested by flow cytometric analysis of ConA stimulated mouse splenocytes using eFluor^TM NC Flow Cytometry Staining Buffer (cat. 00-3222). This can be used at 5 μl per million cells in a 100 μl total staining volume.

The isotype control eFluor^TM 605^NC Armenian hamster Ig (cat. 93-4732) should be used at 5μl/test.

Laser/Filter Recommendation: When using eFluor^TM 605^NC, we recommend excitation with the 405nm violet laser with an appropriate filter set, such as the 595LP dichroic mirror with the 605/40 bandpass filter. An acceptable alternative is the 610/20 bandpass filter. Please contact eBioscience Technical Support for more information.

Buffer Recommendation: Comparison of eFluor^TM Nanocrystal conjugated antibody staining in different buffers has demonstrated that optimal performance is seen with the eFluor^TM NC Flow Cytometry Staining Buffer (cat. 00-3222). For a comparison of staining with different buffers, click here.

Whole Blood Recommendation: If staining whole blood samples, collection into heparin or sodium citrate-containing tubes is recommended. If EDTA is being used as an anti-coagulant, lysis of RBCs with eBioscience 1X RBC Lysis Buffer (cat. 00-4333) before staining is required.

Click here to view our list of eFluor^TM Nanocrystal Frequently Asked Questions.

References

Yokoyama, W. M., F. Koning, et al. (1988). "Characterization of a cell surface-expressed disulfide-linked dimer involved in murine T cell activation." J Immunol 141(2): 369-76.

Related Products

Cat. 93-4888 eFluor™ 605NC Armenian Hamster IgG Isotype Control (clone eBio299Arm)