Product Information

Contents: eFluor^TM 605^NC anti-human CD62L (L-selectin)
Catalog Number: 93-0629
Sizes: 25 tests, 100 tests
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: DREG-56 (DREG56)
Isotype: Mouse IgG1, κ
HLDA No.: V S056

Staining of normal human peripheral blood cells with Pacific Blue anti-human CD4 (OKT4) (cat. 57-0048) and eFluor^TM 605^NC Mouse IgG1, Isotype Control (cat. 93-4714) (left) or eFluor^TM 605^NC anti-human CD62L (L-selectin) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0629	Affinity Purified anti-human CD62L (L-selectin)	N/A	N/A	FA FC IH/F WB
16-0629	Functional Grade* Purified anti-human CD62L (L-selectin)	N/A	N/A	FA FC
11-0629	FITC anti-human CD62L (L-selectin)	488	518	FC
12-0629	PE anti-human CD62L (L-selectin)	488	575	FC
15-0629	PE-Cy5 anti-human CD62L (L-selectin)	488	670	FC
17-0629	Allophycocyanin (APC) anti-human CD62L (L-selectin)	633	660	FC
25-0629	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD62L (L-selectin)	488	760	FC
93-0629	eFluor^TM 605^NC anti-human CD62L (L-selectin)	350-500	605	FC
47-0629	Allophycocyanin-eFluor^TM 780 anti-human CD62L (L-selectin) (APC-Alexa Fluor® 750 replacement)	633	780	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The DREG-56 monoclonal antibody reacts with human CD62L, a 76 kDa member of the selectin family. CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells and binds a number of glycosylated, fucosylated, sulfated sialylated glycoproteins including CD34, glycam-1 and MAdCAM-1. These interactions mediate rolling of lymphocytes on activated endothelium at the sites of inflammation and homing of cells to the high endothelial venules (HEV) of peripheral lymphoid tissues.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This DREG-56 (DREG56) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This DREG-56 (DREG56) antibody has been pre-titrated and tested by flow cytometric analysis of ficoll-prepared human PBMCs using eFluor^TM NC Flow Cytometry Staining Buffer (cat. 00-3222). This can be used at 5 μl per million cells in a 100 μl total staining volume.
The isotype control eFluor^TM 605^NC mouse IgG1 (cat. 93-4714) should be used at 5μl/test.

Laser/Filter Recommendation: When using eFluor^TM 605^NC, we recommend excitation with the 405nm violet laser with an appropriate filter set, such as the 595LP dichroic mirror with the 605/40 bandpass filter. An acceptable alternative is the 610/20 bandpass filter. Please contact eBioscience Technical Support for more information.

Buffer Recommendation: Comparison of eFluor^TM Nanocrystal conjugated antibody staining in different buffers has demonstrated that optimal performance is seen with the eFluor^TM NC Flow Cytometry Staining Buffer (cat. 00-3222). For a comparison of staining with different buffers, click here.

Whole Blood Recommendation: If staining whole blood samples, collection into heparin or sodium citrate-containing tubes is recommended. If EDTA is being used as an anti-coagulant, lysis of RBCs with eBioscience 1X RBC Lysis Buffer (cat. 00-4333) before staining is required.

Click here to view our list of eFluor^TM Nanocrystal Frequently Asked Questions.

References

Schlossman, S., L. Bloumsell, et al. eds. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.

Related Products

Cat. 93-4714 eFluor™ 605NC Mouse IgG1, Isotype Control