Product Information

Contents: eFluor^TM 605^NC anti-human CD45 (LCA)
Catalog Number: 93-0459
Sizes: 25 tests, 100 tests
Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: HI30
Isotype: Mouse IgG1, κ
HLDA No.: IV N816

Staining of normal human peripheral blood cells with eFluor™ 605^NC Mouse IgG1, Isotype Control (cat. 93-4714) (blue) or eFluor™ 605^NC anti-human CD45 (HI30) (purple). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0459	Affinity Purified anti-human CD45 (LCA)	N/A	N/A	FC IHC
13-0459	Biotin anti-human CD45 (LCA)	N/A	N/A	FC
11-0459	FITC anti-human CD45 (LCA)	488	518	FC
12-0459	PE anti-human CD45 (LCA)	488	575	FC
15-0459	PE-Cy5 anti-human CD45 (LCA)	488	670	FC
17-0459	Allophycocyanin (APC) anti-human CD45 (LCA)	633	660	FC
19-0459	Cy5 anti-human CD45 (LCA)	633	670	FC
25-0459	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD45 (LCA)	488	760	FC
35-0459	Phycoerythrin-Cy5.5 (PE-Cy5.5) anti-human CD45 (LCA)	488	690	FC
57-0459	Pacific Blue® anti-human CD45 (LCA)	402	421	FC
27-0459	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-human CD45 (LCA) (APC-Cy7 Equivalent)	633	779	FC
45-0459	PerCP-Cy5.5 anti-human CD45 (LCA) (PE-Cy5.5 replacement)	488	690	FC
93-0459	eFluor^TM 605^NC anti-human CD45 (LCA)	350-500	605	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The HI30 monoclonal antibody reacts with all isoforms of human CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This HI30 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This HI30 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells using the eFluor^TM NC Flow Cytometry Staining Buffer (cat. 00-3222). This can be used at 5 μl/test (one million cells in a 100μL total staining volume).

The isotype control eFluor^TM 605^NC mouse IgG1 (cat. 93-4714) should be used at 5μL/test.

Laser/Filter Recommendation: When using eFluor^TM 605^NC, we recommend excitation with the 405nm violet laser with an appropriate filter set, such as the 595LP dichroic mirror with the 605/40 bandpass filter. An acceptable alternative is the 610/20 bandpass filter. Please contact eBioscience Technical Support for more information.

Buffer Recommendation: Comparison of eFluor^TM Nanocrystal conjugated antibody staining in different buffers has demonstrated that optimal performance is seen with the eFluor^TM NC Flow Cytometry Staining Buffer (cat. 00-3222). For a comparison of staining with different buffers, click here.

Whole Blood Recommendation: If staining whole blood samples, collection into heparin or sodium citrate-containing tubes is recommended. If EDTA is being used as an anti-coagulant, lysis of RBCs with eBioscience 1X RBC Lysis Buffer (cat. 00-4333) before staining is required.

Click here to view our list of eFluor^TM Nanocrystal Frequently Asked Questions.

References

Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.

Related Products

Cat. 00-3222 eFluor™ NC Flow Cytometry Staining Buffer
Cat. 93-4714 eFluor™ 605NC Mouse IgG1, Isotype Control