Product Information

Contents: eFluor^TM 605^NC anti-human CD45RA
Catalog Number: 93-0458
Sizes: 25 tests, 100 tests
Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: HI100
Isotype: Mouse IgG2b, κ
HLDA No.: IV N906

Staining of normal human peripheral blood cells with eFluor™ 605^NC Mouse IgG2b Isotype Control (cat. 93-4732) (blue histogram) or eFluor™ 605 ^NC anti-human CD45RA (HI100) (purple histogram). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0458	Affinity Purified anti-human CD45RA	N/A	N/A	FC IHC
13-0458	Biotin anti-human CD45RA	N/A	N/A	FC
11-0458	FITC anti-human CD45RA	488	518	FC
12-0458	PE anti-human CD45RA	488	575	FC
15-0458	PE-Cy5 anti-human CD45RA	488	670	FC
17-0458	Allophycocyanin (APC) anti-human CD45RA	633	660	FC
25-0458	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD45RA	488	760	FC
45-0458	PerCP-Cy5.5 anti-human CD45RA (PE-Cy5.5 replacement)	488	690	FC
93-0458	eFluor^TM 605^NC anti-human CD45RA	350-500	605	FC
48-0458	eFluor^TM 450 anti-human CD45RA (Pacific Blue® replacement)	405	450	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The HI100 monoclonal antibody reacts with human CD45RA, a 220 kDa molecule expressed by subpopulations of CD4⁺ peripheral T lymphocytes, CD8⁺ peripheral T lymphocytes, and B cells. The CD45RA⁺ T cell populations are mainly naive/virgin allowing the use of HI100 mAb as a phenotypic marker to discriminate T cell subsets.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This HI100 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This HI100 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells using eFluor^TM NC Flow Cytometry Staining Buffer. This can be used at 5 μl/test (one million cells in a 100μL total staining volume).

The isotype control eFluor^TM 605^NC mouse IgG2b (cat.93-0458) should be used at 5 μL/test.

Laser/Filter Recommendation: When using eFluor^TM 605^NC, we recommend excitation with the 405nm violet laser with an appropriate filter set, such as the 595LP dichroic mirror with the 605/40 bandpass filter. An acceptable alternative is the 610/20 bandpass filter. Please contact eBioscience Technical Support for more information.

Buffer Recommendation: Comparison of eFluor^TM Nanocrystal conjugated antibody staining in different buffers has demonstrated that optimal performance is seen with the eFluor^TM NC Flow Cytometry Staining Buffer (cat. 00-3222). For a comparison of staining with different buffers, click here.

Whole Blood Recommendation: If staining whole blood samples, collection into heparin or sodium citrate-containing tubes is recommended. If EDTA is being used as an anti-coagulant, lysis of RBCs with eBioscience 1X RBC Lysis Buffer (cat. 00-4333) before staining is required.

Click here to view our list of eFluor^TM Nanocrystal Frequently Asked Questions.

References

Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.

Related Products

Cat. 00-3222 eFluor™ NC Flow Cytometry Staining Buffer
Cat. 93-4732 eFluor™ 605NC Mouse IgG2b Isotype Control