Product Information

Contents: eFluor^TM 605^NC anti-mouse/human CD45R ( B220)
Catalog Number: 93-0452
Sizes: 25 tests, 100 tests
Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: RA3-6B2
Isotype: Rat IgG2a, κ
HLDA No.: N/A

Staining of BALB/c splenocytes with APC anti-mouse CD3e (145-2C11) (cat. 17-0031) and eFluor™ 605^NC Rat IgG2a Isotype Control (cat. 93-4321) (left) or eFluor™ 605^NC anti-mo/hu CD45R (B220) (RA3-6B2) (right). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0452	Affinity Purified anti-mouse/human CD45R (B220)	N/A	N/A	FC IH/F IHC(Paraffin) IP
16-0452	Functional Grade* Purified anti-mouse/human CD45R (B220)	N/A	N/A	FA FC
13-0452	Biotin anti-mouse/human CD45R (B220)	N/A	N/A	FC
11-0452	FITC anti-mouse/human CD45R (B220)	488	518	FC
12-0452	PE anti-mouse/human CD45R (B220)	488	575	FC
15-0452	PE-Cy5 anti-mouse/human CD45R (B220)	488	670	FC
17-0452	APC anti-mouse/human CD45R (B220)	633	660	FC
19-0452	Cy5 anti-mouse/human CD45R (B220)	633	670	FC
25-0452	PE-Cy7 anti-mouse/human CD45R (B220)	488	760	FC
35-0452	PE-Cy5.5 anti-mouse/human CD45R (B220)	488	690	FC
36-0452	Functional Grade* Biotin anti-mouse/human CD45R (B220)	N/A	N/A	FC
51-0452	Alexa Fluor® 647 anti-mouse/human CD45R (B220)	633	668	FC
53-0452	Alexa Fluor® 488 anti-mouse/human CD45R (B220)	488	519	FC
56-0452	Alexa Fluor® 700 anti-mouse/human CD45R (B220) (APC-Cy5.5 Equivalent)	633	723	FC
45-0452	PerCP-Cy5.5 anti-mouse/human CD45R (B220) (PE-Cy5.5 replacement)	488	690	FC
93-0452	eFluor^TM 605^NC anti-mouse/human CD45R ( B220)	350-500	605	FC
95-0452	eFluor^TM 650^NC anti-mouse/human CD45R (B220)	350-500	650	FC
47-0452	Allophycocyanin-eFluor^TM 780 anti-mouse/human CD45R (B220) (APC-Alexa Fluor® 750 replacement)	633	780	FC
48-0452	eFluor^TM 450 anti-mouse/human CD45R (B220) (Pacific Blue® Replacement)	405	450	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The RA3-6B2 monoclonal antibody reacts with exon A-restricted isoform of mouse CD45, a 220 kDa surface molecule. CD45R/B220 epitope is mainly expressed by the B cell lineage from early Pro-B to mature B cells. However, some activated T cells, lymphokine activated killer cells (LAK), NK cell progenitors in the bone marrow, and T cells of the lpr/lpr mutant mouse also express this antigen.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This RA3-6B2 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This RA3-6B2 antibody has been pre-titrated and tested by flow cytometric analysis of mouse splenocytes using eFluor^TM NC Flow Cytometry Staining Buffer (cat. 00-3222). This can be used at 5 μl/test (one million cells in a 100μL total staining volume).

The isotype control eFluor^TM 605^NCrat IgG2b (cat. 93-4321) should be used at 5 μl/test.

Laser/Filter Recommendation: When using eFluor^TM 605^NC, we recommend excitation with the 405nm violet laser with an appropriate filter set, such as the 595LP dichroic mirror with the 605/40 bandpass filter. An acceptable alternative is the 610/20 bandpass filter. Please contact eBioscience Technical Support for more information.

Buffer Recommendation: Comparison of eFluor^TM Nanocrystal conjugated antibody staining in different buffers has demonstrated that optimal performance is seen with the eFluor^TM NC Flow Cytometry Staining Buffer (cat. 00-3222). For a comparison of staining with different buffers, click here.

Whole Blood Recommendation: If staining whole blood samples, collection into heparin or sodium citrate-containing tubes is recommended. If EDTA is being used as an anti-coagulant, lysis of RBCs with eBioscience 1X RBC Lysis Buffer (cat. 00-4333) before staining is required.

Click here to view our list of eFluor^TM Nanocrystal Frequently Asked Questions.

References

Morse, H. C. d., W. F. Davidson, et al. 1982. Abnormalities induced by the mutant gene Ipr: expansion of a unique lymphocyte subset. J Immunol. 129(6): 2612-5.

Coffman, R. L. 1982. Surface antigen expression and immunoglobulin gene rearrangement during mouse pre-B cell development. Immunol Rev. 69: 5-23.

Schuhmann B, Dietrich A, et al. 2005. A role for brain-derived neurotrophic factor in B cell development. J Neuroimmunol. 163(1-2):15-23. (IHC frozen, PubMed)

Monteith CE, Chelack BJ, et al. 1996. Identification of monoclonal antibodies for immunohistochemical staining of feline B lymphocytes in frozen and formalin-fixed paraffin-embedded tissues. Can J Vet Res. 60(3):193-8. (IHC frozen and paraffin, PubMed)

Related Products

Cat. 00-3222 eFluor™ NC Flow Cytometry Staining Buffer
Cat. 93-4321 eFluor™ 605NC Rat IgG2a Isotype Control