Product Information

Contents: eFluor^TM 605^NC anti-mouse CD45 (Leukocyte Common Antigen, Ly-5)
Catalog Number: 93-0451
Sizes: 25 tests, 100 tests
Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 30-F11
Isotype: Rat IgG2b, κ

Staining of BALB/c bone marrow with FITC TER-119 (TER-119) (cat. 11-5921) and eFluor™ 605^NC Rat IgG2b Isotype Control (cat. 93-4031) (left) or eFluor™ 605^NC anti-mouse CD45 (30-F11) (right). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0451	Affinity Purified anti-mouse CD45 (Leukocyte Common Antigen, Ly-5)	N/A	N/A	FA FC IHC IP
16-0451	Functional Grade* Purified anti-mouse CD45 (Leukocyte Common Antigen, Ly-5)	N/A	N/A	FA FC IHC IP
13-0451	Biotin anti-mouse CD45 (Leukocyte Common Antigen, Ly-5)	N/A	N/A	FC
11-0451	FITC anti-mouse CD45 (Leukocyte Common Antigen, Ly-5)	488	518	FC
12-0451	PE anti-mouse CD45 (Leukocyte Common Antigen, Ly-5)	488	575	FC
15-0451	PE-Cy5 anti-mouse CD45 (Leukocyte Common Antigen, Ly-5)	488	670	FC
17-0451	APC anti-mouse CD45 (Leukocyte Common Antigen, Ly-5)	633	660	FC
25-0451	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse CD45 (Leukocyte Common Antigen, Ly-5)	488	760	FC
35-0451	PE-Cy5.5 anti-mouse CD45 (Leukocyte Common Antigen, Ly-5)	488	690	FC
56-0451	Alexa Fluor® 700 anti-mouse CD45 (Leukocyte Common Antigen, Ly-5) (APC-Cy5.5 Equivalent)	633	723	FC
45-0451	PerCP-Cy5.5 anti-mouse CD45 (Leukocyte Common Antigen, Ly-5) (PE-Cy5.5 replacement)	488	690	FC
93-0451	eFluor^TM 605^NC anti-mouse CD45 (Leukocyte Common Antigen, Ly-5)	350-500	605	FC
47-0451	Allophycocyanin-eFluor^TM 780 anti-mouse CD45 (Leukocyte Common Antigen, Ly-5) (APC-Alexa Fluor® 750 Replacement)	633	780	FC
48-0451	eFluor^TM 450 anti-mouse CD45 (Leukocyte Common Antigen, Ly-5) (Pacific Blue® replacement)	405	450	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 30-F11 monoclonal antibody reacts with all isoforms of mouse CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding mature erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 30-F11 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This 30-F11 antibody has been pre-titrated and tested by flow cytometric analysis of mouse splenocytes using eFluor^TM NC Flow Cytometry Staining Buffer (cat. 00-3222). This can be used at 5 μl /test (one million cells in a 100μL total staining volume).

The isotype control eFluor^TM 605^NC mouse IgG2b (cat. 93-4031) should be used at 5μL/test.

Laser/Filter Recommendation: When using eFluor^TM 605^NC, we recommend excitation with the 405nm violet laser with an appropriate filter set, such as the 595LP dichroic mirror with the 605/40 bandpass filter. An acceptable alternative is the 610/20 bandpass filter. Please contact eBioscience Technical Support for more information.

Buffer Recommendation: Comparison of eFluor^TM NC conjugated antibody staining in different buffers has demonstrated that optimal performance is seen with the eFluor^TM NC Flow Cytometry Staining Buffer (cat. 00-3222). For a comparison of staining with different buffers, click here.

Whole Blood Recommendation: If staining whole blood samples, collection into heparin or sodium citrate-containing tubes is recommended. If EDTA is being used as an anti-coagulant, lysis of RBCs with eBioscience 1X RBC Lysis Buffer (cat. 00-4333) before staining is required.

Click here to view our list of eFluor^TM Nanocrystal Frequently Asked Questions.

References

Ledbetter, J. A. and L. A. Herzenberg (1979). "Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens." Immunol Rev 47: 63-90.

Related Products

Cat. 00-3222 eFluor™ NC Flow Cytometry Staining Buffer
Cat. 93-4031 eFluor™ 605NC Rat IgG2b Isotype Control