Product Information

Contents: eFluor^TM 605 ^NC anti-mouse CD31 (PECAM-1)
Catalog Number: 93-0311
Sizes: 25 tests, 100 tests
Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 390
Isotype: Rat IgG2a, κ

Staining of C57Bl/6 splenocytes with APC anti-mo/hu B220 (RA3-6B2) (cat. 17-0452) and eFluor™ 605^NC Rat IgG2a Isotype Control (cat. 93-4321) (left) or eFluor^TM 605^NC anti-mouse CD31 (390) (right). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0311	Affinity Purified anti-mouse CD31 (PECAM-1)	N/A	N/A	FC IH/F IP
16-0311	Functional Grade* Purified anti-mouse CD31 (PECAM-1)	N/A	N/A	FA FC
13-0311	Biotin anti-mouse CD31 (PECAM-1)	N/A	N/A	FC
11-0311	FITC anti-mouse CD31 (PECAM-1)	488	518	FC
12-0311	PE anti-mouse CD31 (PECAM-1)	488	575	FC
17-0311	Allophycocyanin (APC) anti-mouse CD31 (PECAM-1)	633	660	FC
25-0311	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse CD31 (PECAM-1)	488	760	FC
36-0311	Functional Grade* Biotin anti-mouse CD31 (PECAM-1)	N/A	N/A	FC
93-0311	eFluor^TM 605^NC anti-mouse CD31 (PECAM-1)	350-500	605	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Custom Product Requests
Need a custom product? Download the Custom Product Request Form and submit completed form to customs@ebioscience.com.

eFluor products are not offered for sale or use in certain regions. Please contact an eBioscience representative for more information on whether our products are available for purchase in your area.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 390 monoclonal antibody reacts with mouse CD31, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1) and gpIIa. This 130-140 kDa surface protein is expressed by endothelial cells and at low levels on all leukocytes and platelets. It has been reported that CD38 binds to CD31. Homotypic interaction of CD31 is important in adhesion, cell-cell and cell-matrix interaction, and signal transduction.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 390 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This 390 antibody has been pre-titrated and tested by flow cytometric analysis of mouse splenocytes. This can be used at 5 μl per million cells in a 100 μl total staining volume.

The isotype control eFluor^TM 605^NC rat IgG2a (cat. 93-4321) should be used at 5μL/test.

Laser/Filter Recommendation: When using eFluor^TM 605^NC, we recommend excitation with the 405nm violet laser with an appropriate filter set, such as the 595LP dichroic mirror with the 605/40 bandpass filter. An acceptable alternative is the 610/20 bandpass filter. Please contact eBioscience Technical Support for more information.

Buffer Recommendation: Comparison of eFluor^TM Nanocrystal conjugated antibody staining in different buffers has demonstrated that optimal performance is seen with the eFluor^TM NC Flow Cytometry Staining Buffer (cat. 00-3222). For a comparison of staining with different buffers, click here.

Whole Blood Recommendation: If staining whole blood samples, collection into heparin or sodium citrate-containing tubes is recommended. If EDTA is being used as an anti-coagulant, lysis of RBCs with eBioscience 1X RBC Lysis Buffer (cat. 00-4333) before staining is required.

Click here to view our list of eFluor^TM Nanocrystal Frequently Asked Questions.

References

Sadri N, Schneider RJ. Auf1/Hnrnpd-Deficient Mice Develop Pruritic Inflammatory Skin Disease. J Invest Dermatol. 2008 Oct 2. [Epub ahead of print]. (390, IHC frozen, PubMed)

Cao G, O'Brien CD, Zhou Z, Sanders SM, Greenbaum JN, Makrigiannakis A, DeLisser HM. Involvement of human PECAM-1 in angiogenesis and in vitro endothelial cell migration. Am J Physiol Cell Physiol. 2002 May;282(5):C1181-90. (390, IHC frozen)

Wiewrodt R, Anu P. Thomas, Luca Cipelletti, Melpo Christofidou-Solomidou, David A. Weitz, Sheldon I. Feinstein, David Schaffer, Steven M. Albelda, Michael Koval, and Vladimir R. Muzykantov. Size-dependent intracellular immunotargeting of therapeutic cargoes into endothelial cells.Blood. 2002 Feb 1;99(3):912-22.

Horenstein AL, Stockinger H, Imhof BA, Malavasi F. CD38 binding to human myeloid cells is mediated by mouse and human CD31. Biochem J. 1998 Mar 15;330 ( Pt 3):1129-35.

Baldwin HS, Shen HM, Yan HC, DeLisser HM, Chung A, Mickanin C, Trask T, Kirschbaum NE, Newman PJ, Albelda SM, et al. Platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31): alternatively spliced, functionally distinct isoforms expressed during mammalian cardiovascular development. Development. 1994 Sep;120(9):2539-53.

Related Products

Cat. 93-4321 eFluor™ 605NC Rat IgG2a Isotype Control