human CD19

Contents: eFluor^TM 605^NC anti-human CD19
Catalog Number: 93-0199
Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: HIB19
Isotype: Mouse IgG1, κ
HLDA No.: V CD19.11

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
93-0199-71	25 tests	$145
93-0199-73	100 tests	$330

*International customers: Please contact your distributor for region specific pricing.

Staining of normal human peripheral blood cells with Pacific Blue anti-human CD3 (UCHT1) (cat. 57-0038) and eFluor™ 605^NC Mouse IgG1, Isotype Control (cat. 93-4714) (left) or eFluor™ 605^NC anti-human CD19 (HIB19) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0199	Affinity Purified anti-human CD19	N/A	N/A	FA FC IH/F
16-0199	Functional Grade* Purified anti-human CD19	N/A	N/A	FA FC
13-0199	Biotin anti-human CD19	N/A	N/A	FC
11-0199	FITC anti-human CD19	488	518	FC
12-0199	PE anti-human CD19	488	575	FC
15-0199	PE-Cy5 anti-human CD19	488	670	FC
17-0199	APC anti-human CD19	633	660	FC
25-0199	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD19	488	760	FC
51-0199	Alexa Fluor® 647 anti-human CD19	633	668	FC
53-0199	Alexa Fluor® 488 anti-human CD19	488	519	FC
56-0199	Alexa Fluor® 700 anti-human CD19 (APC-Cy5.5 Equivalent)	633	723	FC
57-0199	Pacific Blue® anti-human CD19	402	421	FC
27-0199	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-human CD19 (APC-Cy7 Equivalent)	633	779	FC
45-0199	PerCP-Cy5.5 anti-human CD19	488	690	FC
93-0199	eFluor^TM 605^NC anti-human CD19	350-500	605	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

eFluor products are not offered for sale or use in certain regions. Please contact an eBioscience representative for more information on whether our products are available for purchase in your area.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The HIB19 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, Leu13, MHC class II, and CD19 form a multimolecular complex that associates with BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This HIB19 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This HIB19 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood using eFluor^TM NC Flow Cytometric Staining Buffer (cat. 00-3222). This can be used at 5 μl /test (one million cells in a 100μL total staining volume).

The isotype control eFluor^TM 605^NCmouse IgG1 (cat. 93-4714) should be used at 2.5 μL/test.

Laser/Filter Recommendation: When using eFluor^TM 605^NC, we recommend excitation with the 405nm violet laser with an appropriate filter set, such as the 595LP dichroic mirror with the 605/40 bandpass filter. An acceptable alternative is the 610/20 bandpass filter. Please contact eBioscience Technical Support for more information.

Buffer Recommendation: Comparison of eFluor^TM Nanocrystal conjugated antibody staining in different buffers has demonstrated that optimal performance is seen with the eFluor^TM NC Flow Cytometry Staining Buffer (cat. 00-3222). For a limited time the eFluor^TM NC Flow Cytometry Staining Buffer will be provided complimentary with the purchase of eFluor^TM Nanocrystal conjugated products. For a comparison of staining with different buffers, click here.

Whole Blood Recommendation: If staining whole blood samples, collection into heparin or sodium citrate-containing tubes is recommended. If EDTA is being used as an anti-coagulant, lysis of RBCs with eBioscience 1X RBC Lysis Buffer (cat. 00-4333) before staining is required.

Click here to view our list of eFluor^TM Nanocrystal Frequently Asked Questions.

References

Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
Schlossman, S., L. Bloumsell, et al. eds (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.

Related Products

Cat. 00-3222 eFluor™ NC Flow Cytometry Staining Buffer
Cat. 93-4714 eFluor™ 605NC Mouse IgG1, Isotype Control