Product Information

Contents: eFluor^TM 605^NC anti-human CD14
Catalog Number: 93-0149
Sizes: 25 tests, 100 tests
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 61D3
Isotype: Mouse IgG1, κ
HLDA No.: V MA085

Staining of normal human peripheral blood cells with eFluor^TM 605^NC Mouse IgG1, Isotype Control (cat. 93-4714) (blue histogram) or eFluor^TM 605^NC anti-human CD14 (purple histogram). Cells in the monocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0149	Affinity Purified anti-human CD14	N/A	N/A	FA FC IHC
16-0149	Functional Grade* Purified anti-human CD14	N/A	N/A	FA FC
13-0149	Biotin anti-human CD14	N/A	N/A	FC
11-0149	FITC anti-human CD14	488	518	FC
12-0149	PE anti-human CD14	488	575	FC
17-0149	APC anti-human CD14	633	660	FC
25-0149	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD14	488	760	FC
27-0149	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-human CD14	633	779	FC
45-0149	PerCP-Cy5.5 anti-human CD14 (PE-Cy5.5 replacement)	488	690	FC
93-0149	eFluor^TM 605^NC anti-human CD14	350-500	605	FC
48-0149	eFluor^TM 450 anti-human CD14 (Pacific Blue® replacement)	405	450	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 61D3 monoclonal antibody reacts with human CD14, a 53-55 kDa GPI-linked glycoprotein. CD14 is expressed on monocytes, interfollicular macrophages and some dendritic cells. Complexes of LPS and LBP (LPS-Binding Protein) bind with high affinity to monocytes through the surface CD14.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 61D3 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This 61D3 antibody has been pre-titrated and tested by flow cytometric analysis of Ficoll prepared human PBMCs and staining using eFluor^TM NC Flow Cytometry Staining Buffer (cat. 00-3222). This can be used at 5 μl per million cells in a 100 μl total staining volume.

The isotype control eFluor^TM 605^NC mouse IgG1 (cat. 93-4714) should be used at 5μl/test.

Laser/Filter Recommendation: When using eFluor^TM 605^NC, we recommend excitation with the 405nm violet laser with an appropriate filter set, such as the 595LP dichroic mirror with the 605/40 bandpass filter. An acceptable alternative is the 610/20 bandpass filter. Please contact eBioscience Technical Support for more information.

Buffer Recommendation: Comparison of eFluor^TM Nanocrystal conjugated antibody staining in different buffers has demonstrated that optimal performance is seen with the eFluor^TM NC Flow Cytometry Staining Buffer (cat. 00-3222). For a comparison of staining with different buffers, click here.

Whole Blood Recommendation: If staining whole blood samples, collection into heparin or sodium citrate-containing tubes is recommended. If EDTA is being used as an anti-coagulant, lysis of RBCs with eBioscience 1X RBC Lysis Buffer (cat. 00-4333) before staining is required.

Click here to view our list of eFluor^TM Nanocrystal Frequently Asked Questions.

References

Schlossman, S., L. Bloumsell, et al. eds (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.
Kishimoto, T., A.E.G., von dem Borne, et al. eds. (1998). Leucocyte Typing VI: White Cell Differentiation Antigens. Garland Publishing Inc. London.
Fadok VA, Warner ML, Bratton DL, Henson PM. CD36 is required for phagocytosis of apoptotic cells by human macrophages that use either a phosphatidylserine receptor or the vitronectin receptor (alpha v beta 3). J Immunol 1998 Dec 1;161(11):6250-7.

Related Products

Cat. 93-4714 eFluor™ 605NC Mouse IgG1, Isotype Control