human CD11b (Integrin alpha M, Mac-1 alpha)

Contents: eFluor^TM 605^NC anti-human CD11b(Integrin alpha M, Mac-1 alpha)
Catalog Number: 93-0118
Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: ICRF44
Isotype: Mouse IgG1, κ
HLDA No.: IV M047

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
93-0118-71	25 tests	$145
93-0118-73	100 tests	$330

*International customers: Please contact your distributor for region specific pricing.

Staining of normal human peripheral blood cells with eFluor™ 605^NC Mouse IgG1, Isotype Control (cat. 93-4714) (blue) or eFluor™ 605^NC anti-human CD11b (ICRF44) (purple). Cells in the monocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0118	Affinity Purified anti-human CD11b (Integrin alpha M, Mac-1 alpha)	N/A	N/A	FA FC IHC
13-0118	Biotin anti-human CD11b (Integrin alpha M, Mac-1 alpha)	N/A	N/A	FC
11-0118	Fluorescein isothiocyanate (FITC) anti-human CD11b (Integrin alpha M, Mac-1 alpha)	488	518	FC
12-0118	PE anti-human CD11b (Integrin alpha M, Mac-1 alpha)	488	575	FC
17-0118	Allophycocyanin (APC) anti-human CD11b (Integrin alpha M, Mac-1 alpha)	633	660	FC
25-0118	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD11b (Integrin alpha M, Mac-1 alpha)	488	760	FC
93-0118	eFluor^TM 605^NC anti-human CD11b(Integrin alpha M, Mac-1 alpha)	350-500	605	FC

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Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The ICRF44 monoclonal antibody reacts with human CD11b, a 165 kDa adhesion molecule. CD11b associated with integrin β₂ (CD18) is expressed on the surface of monocytes, granulocytes, activated lymphocytes and a subset of NK cells. CD11b is a receptor for intercellular adhesion molecule family members CD54, CD102 and CD50 as well as for iC3b. These adhesions are crucial in cell-cell and cell-matrix interactions.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This ICRF44 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This ICRF44 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells (after RBC lysis) using the eFluor^TM NC Flow Cytometry Staining Buffer (cat. 00-3222). This can be used at 5 μl /test (one million cells in a 100μL total staining volume).

The isotype control eFluor^TM 605^NC mouse IgG21 (cat. 93-4714) should be used at 5μl/test.

Laser/Filter Recommendation: When using eFluor^TM 605^NC, we recommend excitation with the 405nm violet laser with an appropriate filter set, such as the 595LP dichroic mirror with the 605/40 bandpass filter. An acceptable alternative is the 610/20 bandpass filter. Please contact eBioscience Technical Support for more information.

Buffer Recommendation: Comparison of eFluor^TM Nanocrystal conjugated antibody staining in different buffers has demonstrated that optimal performance is seen with the eFluor^TM NC Flow Cytometry Staining Buffer (cat. 00-3222). For a limited time the eFluor^TM NC Flow Cytometry Staining Buffer will be provided complimentary with the purchase of eFluor^TM Nanocrystal conjugated products. For a comparison of staining with different buffers, click here.

Whole Blood Recommendation: If staining whole blood samples, collection into heparin or sodium citrate-containing tubes is recommended. If EDTA is being used as an anti-coagulant, lysis of RBCs with eBioscience 1X RBC Lysis Buffer (cat. 00-4333) before staining is required.

Click here to view our list of eFluor^TM Nanocrystal Frequently Asked Questions.

References

Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.

Related Products

Cat. 00-3222 eFluor™ NC Flow Cytometry Staining Buffer
Cat. 93-4714 eFluor™ 605NC Mouse IgG1, Isotype Control