Product Information

Contents: eFluor^TM 605^NC anti-mouse CD4 (L3T4)
Catalog Number: 93-0041
Sizes: 25 tests, 100 tests
Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: GK1.5
Isotype: Rat IgG2b, κ

Staining of BALB/c thymocytes with Pacific Blue anti-mouse CD8 (53-6.7) (cat. 57-0081) and eFluor™ 605^NC Rat IgG2b Isotype Control (cat. 93-4031) (left) or eFluor™ 605^NC anti-mouse CD4 (GK1.5) (right). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0041	Affinity Purified anti-mouse CD4 (L3T4)	N/A	N/A	FC IH/F IP
16-0041	Functional Grade* Purified anti-mouse CD4 (L3T4)	N/A	N/A	FA FC
13-0041	Biotin anti-mouse CD4 (L3T4)	N/A	N/A	FC
11-0041	FITC anti-mouse CD4 (L3T4)	488	518	FC
12-0041	PE anti-mouse CD4 (L3T4)	488	575	FC
15-0041	PE-Cy5 anti-mouse CD4 (L3T4)	488	670	FC
17-0041	APC anti-mouse CD4 (L3T4)	633	660	FC
19-0041	Cy5 anti-mouse CD4 (L3T4)	633	670	FC
25-0041	PE-Cy7 anti-mouse CD4 (L3T4)	488	760	FC
36-0041	Functional Grade* Biotin anti-mouse CD4 (L3T4)	N/A	N/A	FC IH/F IP
51-0041	Alexa Fluor® 647 anti-mouse CD4 (L3T4)	633	668	FC
53-0041	Alexa Fluor® 488 anti-mouse CD4 (L3T4)	488	519	FC
56-0041	Alexa Fluor® 700 anti-mouse CD4 (L3T4) (APC-Cy5.5 Equivalent)	633	723	FC
93-0041	eFluor^TM 605^NC anti-mouse CD4 (L3T4)	350-500	605	FC
95-0041	eFluor^TM 650^NC anti-mouse CD4 (L3T4)	350-500	650	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The GK1.5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of GK1.5 is blocked by RM4-5.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This GK1.5 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This GK1.5 antibody has been pre-titrated and tested by flow cytometric analysis of mouse splenocytes using the eFluor^TM NC Flow Cytometry Staining Buffer (cat. 00-3222). This should be used at 5 ul/test (one million cells in a 100 ul total staining volume).

The isotype control eFluor^TM 605^NC mouse IgG2b (cat. 93-4732) should be used at 5μl/test.

Laser/Filter Recommendation: When using eFluor^TM 605^NC, we recommend excitation with the 405nm violet laser with an appropriate filter set, such as the 595LP dichroic mirror with the 605/40 bandpass filter. An acceptable alternative is the 610/20 bandpass filter. Please contact eBioscience Technical Support for more information.

Buffer Recommendation: Comparison of eFluor^TM Nanocrystal conjugated antibody staining in different buffers has demonstrated that optimal performance is seen with the eFluor^TM NC Flow Cytometry Staining Buffer (cat. 00-3222). For a comparison of staining with different buffers, click here.

Whole Blood Recommendation: If staining whole blood samples, collection into heparin or sodium citrate-containing tubes is recommended. If EDTA is being used as an anti-coagulant, lysis of RBCs with eBioscience 1X RBC Lysis Buffer (cat. 00-4333) before staining is required.

Click here to view our list of eFluor^TM Nanocrystal Frequently Asked Questions.

References

Mochimaru H, Usui T, Yaguchi T, Nagahama Y, Hasegawa G, Usui Y, Shimmura S, Tsubota K, Amano S, Kawakami Y, Ishida S. Suppression of alkali burn-induced corneal neovascularization by dendritic cell vaccination targeting VEGF receptor 2. Invest Ophthalmol Vis Sci. 2008 May;49(5):2172-7. (GK1.5, in vivo depletion, PubMed)

Yang Z, Day YJ, Toufektsian MC, Xu Y, Ramos SI, Marshall MA, French BA, Linden J. Myocardial infarct-sparing effect of adenosine A2A receptor activation is due to its action on CD4+ T lymphocytes. Circulation. 2006 Nov 7;114(19):2056-64. (GK1.5, in vivo depletion, PubMed)

Dialynas DP, Quan ZS, Wall KA, Pierres A, Quintáns J, Loken MR, Pierres M, Fitch FW. Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody GK1.5: similarity of L3T4 to the human Leu-3/T4 molecule. J Immunol. 1983 Nov;131(5):2445-51.

Wilde DB, Marrack P, Kappler J, Dialynas DP, Fitch FW. Evidence implicating L3T4 in class II MHC antigen reactivity; monoclonal antibody GK1.5 (anti-L3T4a) blocks class II MHC antigen- specific proliferation, release of lymphokines, and binding by cloned murine helper T lymphocyte lines. J Immunol. 1983 Nov;131(5):2178-83.

Related Products

Cat. 00-3222 eFluor™ NC Flow Cytometry Staining Buffer
Cat. 93-4031 eFluor™ 605NC Rat IgG2b Isotype Control