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eFluor products are not offered for sale or use in certain regions. Please contact an eBioscience representative for more information on whether our products are available for purchase in your area. Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq. DescriptionThe GK1.5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of GK1.5 is blocked by RM4-5. Applications ReportedFor research use only, not for diagnostic or therapeutic use. This GK1.5 antibody has been reported for use in flow cytometric analysis. Applications TestedThis GK1.5 antibody has been pre-titrated and tested by flow cytometric analysis of mouse splenocytes using the eFluorTM NC Flow Cytometry Staining Buffer (cat. 00-3222). This should be used at 5 ul/test (one million cells in a 100 ul total staining volume). The isotype control eFluorTM 605NC mouse IgG2b (cat. 93-4732) should be used at 5μl/test. Laser/Filter Recommendation: When using eFluorTM 605NC, we recommend excitation with the 405nm violet laser with an appropriate filter set, such as the 595LP dichroic mirror with the 605/40 bandpass filter. An acceptable alternative is the 610/20 bandpass filter. Please contact eBioscience Technical Support for more information. Buffer Recommendation: Comparison of eFluorTM Nanocrystal conjugated antibody staining in different buffers has demonstrated that optimal performance is seen with the eFluorTM NC Flow Cytometry Staining Buffer (cat. 00-3222). For a limited time the eFluorTM NC Flow Cytometry Staining Buffer will be provided complimentary with the purchase of eFluorTM Nanocrystal conjugated products. For a comparison of staining with different buffers, click here. Whole Blood Recommendation: If staining whole blood samples, collection into heparin or sodium citrate-containing tubes is recommended. If EDTA is being used as an anti-coagulant, lysis of RBCs with eBioscience 1X RBC Lysis Buffer (cat. 00-4333) before staining is required. Click here to view our list of eFluorTM Nanocrystal Frequently Asked Questions. References
Mochimaru H, Usui T, Yaguchi T, Nagahama Y, Hasegawa G, Usui Y, Shimmura S, Tsubota K, Amano S, Kawakami Y, Ishida S. Suppression of alkali burn-induced corneal neovascularization by dendritic cell vaccination targeting VEGF receptor 2. Invest Ophthalmol Vis Sci. 2008 May;49(5):2172-7. (GK1.5, in vivo depletion, PubMed) Yang Z, Day YJ, Toufektsian MC, Xu Y, Ramos SI, Marshall MA, French BA, Linden J. Myocardial infarct-sparing effect of adenosine A2A receptor activation is due to its action on CD4+ T lymphocytes. Circulation. 2006 Nov 7;114(19):2056-64. (GK1.5, in vivo depletion, PubMed) Dialynas DP, Quan ZS, Wall KA, Pierres A, Quintáns J, Loken MR, Pierres M, Fitch FW. Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody GK1.5: similarity of L3T4 to the human Leu-3/T4 molecule. J Immunol. 1983 Nov;131(5):2445-51. Wilde DB, Marrack P, Kappler J, Dialynas DP, Fitch FW. Evidence implicating L3T4 in class II MHC antigen reactivity; monoclonal antibody GK1.5 (anti-L3T4a) blocks class II MHC antigen- specific proliferation, release of lymphokines, and binding by cloned murine helper T lymphocyte lines. J Immunol. 1983 Nov;131(5):2178-83. Related ProductsCat. 00-3222 eFluor™ NC Flow Cytometry Staining Buffer Cat. 93-4031 eFluor™ 605NC Rat IgG2b Isotype Control |
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Mouse Cellular Antigens Chart