Product Information

Contents: eFluor^TM 605^NC anti-human CD3
Catalog Number: 93-0037
Sizes: 25 tests, 100 tests
Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: OKT3
Isotype: Mouse IgG2a
HLDA No.: VI T6T-R3

Staining of normal human peripheral blood cells with APC anti-human CD19 (HIB19) (cat. 17-0199) and eFluor™ 605^NC Mouse IgG2a, Isotype Control (cat. 93-4724) (left) or eFluor^TM 605^NC anti-human CD3 (OKT-3) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0037	Affinity Purified anti-human CD3	N/A	N/A	FC
16-0037	Functional Grade* Purified anti-human CD3	N/A	N/A	FA FC
13-0037	Biotin anti-human CD3	N/A	N/A	FC
11-0037	FITC anti-human CD3	488	518	FC
12-0037	PE anti-human CD3	488	575	FC
51-0037	Alexa Fluor® 647 anti-human CD3	633	668	FC
53-0037	Alexa Fluor® 488 anti-human CD3	488	519	FC
56-0037	Alexa Fluor® 700 anti-human CD3 (APC-Cy5.5 Equivalent)	633	723	FC
45-0037	PerCP-Cy5.5 anti-human CD3 (PE-Cy5.5 replacement)	488	690	FC
93-0037	eFluor^TM 605^NC anti-human CD3	350-500	605	FC
95-0037	eFluor^TM 650^NC anti-human CD3	350-500	650	FC
48-0037	eFluor^TM 450 anti-human CD3 (Pacific Blue® replacement)	405	450	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex. The OKT3 antibody has been reported to have potent immunosuppressive properties in vivo and has been proven effective in the treatment of renal, heart and liver allograft rejection. The CD3 subunits, γ, δ, and ε chains, are required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR initiates an intracellular biochemical pathway resulting in cellular activation and proliferation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This OKT3 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This OKT3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells using eFluor NC Flow Staining Buffer (cat. 00-3222). This can be used at 5 μl per million cells in a 100 μl total staining volume.

The isotype control eFluor^TM 605^NC mouse IgG2a (cat. 93-4724) should be used at 5μL/test.

Laser/Filter Recommendation: When using eFluor^TM 605^NC, we recommend excitation with the 405nm violet laser with an appropriate filter set, such as the 595LP dichroic mirror with the 605/40 bandpass filter. An acceptable alternative is the 610/20 bandpass filter. Please contact eBioscience Technical Support for more information.

Buffer Recommendation: Comparison of eFluor^TM Nanocrystal conjugated antibody staining in different buffers has demonstrated that optimal performance is seen with the eFluor^TM NC Flow Cytometry Staining Buffer (cat. 00-3222). For a comparison of staining with different buffers, click here.

Whole Blood Recommendation: If staining whole blood samples, collection into heparin or sodium citrate-containing tubes is recommended. If EDTA is being used as an anti-coagulant, lysis of RBCs with eBioscience 1X RBC Lysis Buffer (cat. 00-4333) before staining is required.

Click here to view our list of eFluor^TM Nanocrystal Frequently Asked Questions.

References

Hoffman RA et al. Simple and rapid measurement of human T lymphocytes and their subclasses in peripheral blood. Proc. Natl. Acad. Sci. USA 77: 4914-4917, 1980. PubMed: 81054881
Kung PC and Goldstein G. Complement-fixing monoclonal antibody to human T cells, and methods of preparing same. U.S. Pat. 4,361,549 dated Nov. 30, 1982.
Tanaka Y et al. Productive and lytic infection of human CD4+ type 1 helper T cells with macrophage-tropic human immunodeficiency virus type 1. J. Virol. 71: 465-470, 1997. PubMed: 97138346
Solomon KR et al. The association between glycoslphosphatidylinositol-anchored proteins and hetrotrimeric G protein alpha subunits in lymphocytes. Proc. Natl. Acad. Sci. USA 93: 6053-6058, 1996. PubMed: 96234088
Hutchcroft JE et al. Differential phosphorylation of the T lymphocyte costimulatory receptor CD28. J. Biol. Chem. 271: 13362-13370, 1996. PubMed: 96278754
Adair, JR., et al. 1994. Humanization of the murine anti-human CD3 monoclonal antibody OKT3. Hum Antibodies Hybridomas. 5:41-7.

Related Products

Cat. 93-4724 eFluor™ 605NC Mouse IgG2a, Isotype Control